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An immunocompetent mouse model for the tolerance of human chronic hepatitis B virus infection.
- Source :
-
Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2006 Nov 21; Vol. 103 (47), pp. 17862-7. Date of Electronic Publication: 2006 Nov 09. - Publication Year :
- 2006
-
Abstract
- An animal model for human hepatitis B virus (HBV) tolerance is needed to investigate the mechanisms. This model will also facilitate therapeutic strategies for the existing 350 million patients with chronic hepatitis B. We established a mouse model by hydrodynamic injection of an engineered, replication-competent HBV DNA into the tail veins of C57BL/6 mice. In 40% of the injected mice, HBV surface antigenemia persisted for > 6 months. Viral replication intermediates, transcripts, and proteins were detected in the liver tissues of the injected mice for up to 1 year. The tolerance toward HBV surface antigen in this model was shown to be due to an insufficient cellular immunity against hepatitis B core antigen, as was documented in humans. This animal model will accelerate further genetic and mechanistic studies of human chronic hepatitis B infection.
- Subjects :
- Animals
Antigens, Viral immunology
Disease Models, Animal
Humans
Interferon-gamma immunology
Liver cytology
Liver pathology
Liver virology
Male
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Plasmids administration & dosage
Plasmids genetics
Hepatitis B virus immunology
Hepatitis B, Chronic immunology
Immunocompetence physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0027-8424
- Volume :
- 103
- Issue :
- 47
- Database :
- MEDLINE
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Publication Type :
- Academic Journal
- Accession number :
- 17095599
- Full Text :
- https://doi.org/10.1073/pnas.0608578103