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Influence of wine pectic polysaccharides on the interactions between condensed tannins and salivary proteins.
- Source :
-
Journal of agricultural and food chemistry [J Agric Food Chem] 2006 Nov 15; Vol. 54 (23), pp. 8936-44. - Publication Year :
- 2006
-
Abstract
- Alpha-amylase, a major human salivary protein, and IB8c, a representative of the proline-rich proteins, were obtained by isolation from saliva and by solid-phase synthesis, respectively. The interactions between these proteins and condensed tannins isolated from grape seeds were studied at different protein and tannin concentrations by measuring their aggregation. Pectic polysaccharides were isolated from wine, and their effect on protein tannin aggregation was assessed. The results presented in this study showed that the most acidic fractions of arabinogalactan proteins have the ability to inhibit the formation of aggregates between the grape seed tannins and the two different salivary proteins. Rhamnogalacturonan II has the same ability toward alpha-amylase but not IB8c under the conditions of the present study. Polysaccharides show effects at concentrations at which they are present in wine, which could mean an influence in wine astringency. The interaction between condensed tannins and alpha-amylase is differently affected by ionic strength when compared with IB8c.
- Subjects :
- Amino Acid Sequence
Light
Molecular Sequence Data
Peptides chemistry
Proline-Rich Protein Domains
Scattering, Radiation
Seeds chemistry
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Vitis chemistry
Pectins chemistry
Polysaccharides chemistry
Salivary Proteins and Peptides chemistry
Tannins chemistry
Wine
alpha-Amylases chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 0021-8561
- Volume :
- 54
- Issue :
- 23
- Database :
- MEDLINE
- Journal :
- Journal of agricultural and food chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 17090144
- Full Text :
- https://doi.org/10.1021/jf061835h