Back to Search
Start Over
Chromosomal breakpoints affecting immunoglobulin loci are recurrent in Hodgkin and Reed-Sternberg cells of classical Hodgkin lymphoma.
- Source :
-
Cancer research [Cancer Res] 2006 Nov 01; Vol. 66 (21), pp. 10332-8. - Publication Year :
- 2006
-
Abstract
- Chromosomal breakpoints affecting immunoglobulin (IG) loci are recurrent in many subtypes of B-cell lymphomas. However, despite the predominant B-cell origin of the Hodgkin and Reed-Sternberg (HRS) cells in classical Hodgkin lymphoma (cHL), the presence of chromosomal translocations in IG loci has not yet been systematically explored. Therefore, we have investigated a series of cHL for chromosomal breakpoints in the IGH (n = 230), IGL (n = 139), and IGK (n = 138) loci by interphase cytogenetics. Breakpoints in the IGH, IGL, or IGK locus were observed in the HRS cells of 26 of 149 (17%), 2 of 70, and 1 of 77 evaluable cHLs, respectively. The IG partners could be identified in eight cHLs and involved chromosomal bands 2p16 (REL), 3q27 (BCL6, two cases), 8q24.1 (MYC), 14q24.3, 16p13.1, 17q12, and 19q13.2 (BCL3/RELB). In 65 of 85 (76%) cHLs evaluable for an IGH triple-color probe, the HRS cells showed evidence for a (partial) deletion of the IGH constant region, suggesting the presence of class switch recombination (CSR). Furthermore, analyses with this probe in cases with IGH breakpoints indicated that at least part of them seem to be derived from CSR defects. Our results show that chromosomal breakpoints affecting the IG loci are recurrent in cHL.
- Subjects :
- Adolescent
Adult
Aged
Caspases genetics
DNA-Binding Proteins genetics
Female
Genes, myc
Humans
Immunoglobulin Class Switching
Immunoglobulin Constant Regions genetics
In Situ Hybridization, Fluorescence
Male
Middle Aged
Mucosa-Associated Lymphoid Tissue Lymphoma Translocation 1 Protein
Neoplasm Proteins genetics
Proto-Oncogene Proteins c-bcl-6
Recurrence
Translocation, Genetic
Chromosome Breakage
Genes, Immunoglobulin Heavy Chain
Hodgkin Disease genetics
Reed-Sternberg Cells metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0008-5472
- Volume :
- 66
- Issue :
- 21
- Database :
- MEDLINE
- Journal :
- Cancer research
- Publication Type :
- Academic Journal
- Accession number :
- 17079453
- Full Text :
- https://doi.org/10.1158/0008-5472.CAN-06-1992