[Alterations of intermedin and its receptor system in oleic acid-induced acute lung injury of rats].

Objective: To investigate the changes of pulmonary IMD and its receptor system-calcitonin receptor-like receptor (CL) and receptor activity modifying proteins (RAMPs) mRNA in acute lung injury(ALI) induced by oleic acid of rats.
Methods: Contents of IMD in plasma and lung homogenates were measured by radioimmunoassay(RIA). The lung mRNA of IMD, CL and RAMPs was determined by semi-quantitative RT-PCR.
Results: Compared with control group, in ALI group, the contents of IMD1-53 in plasma and lung homogenates were decreased by 20.8% and 74.5% (all P < 0.05) , respectively. Furthermore , it was found that the levels of IMD, CL, RAMP1 and RAMP2 mRNA in lung were decreased by 30%, 38%, 26% and 37.9% (all P < 0. 05) , respectively. The levels of CL , RAMP1 or RAMP2 mRNA were positively correlated with down-regulations of IMD mRNA in ALI. However, compared with control group, the maximum binding capacity of IMD1-53 to plasma membranes was significantly increased in ALI group, and the affinity of IMD1-53 for its receptor almost had no change.
Conclusion: The amount of IMD 1-53 is down-regulated and IMD receptor system also down-regulated in Oliec acid induced ALI of rats. These changes suggest that IMD and its receptor system probably are involved in the development of ALI.