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Enhanced liver metabolism of mutagens and carcinogens in fish living in polluted seawater.
- Source :
-
Mutation research [Mutat Res] 1991 Feb; Vol. 262 (2), pp. 129-37. - Publication Year :
- 1991
-
Abstract
- Specimens of the seawater fish annular seabream (Diplodus annularis) were caught from a polluted harbor area and from a clean reference area. Seawater concentrates and fish-muscle extracts were not mutagenic in the Salmonella reversion test. Liver preparations of fish from the 2 sources were comparatively assayed for microsomal mixed-function oxidases and cytosolic biochemical parameters, as well as for the ability of S12 fractions to activate promutagens or to detoxify direct-acting mutagens. A shift of the cytochrome P-450 peak from 450.3 to 448.5 was accompanied by a 4.5-fold increase in arylhydrocarbon hydroxylase activity in fish living in the polluted environment. At the same time, glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase were doubled in the cytosol of the same animals, while reduced glutathione (GSH) peroxidase and GSH S-transferase were slightly yet significantly depressed. No significant difference was recorded for other biochemical parameters, including GSH, oxidized glutathione (GSSG) reductase, NADH- and NADPH-dependent diaphorases, and DT diaphorase. In parallel, fish exposed to polluted seawater exhibited a significant and marked enhancement of the metabolic activation of the pyrolysis product Trp-P-2 and of benzo[a]pyrene-trans-7,8-diol, and at the same time were less efficient in detoxifying the antitumor compound ICR 191. Liver S12 fractions from both sources efficiently decreased the direct mutagenicity of sodium dichromate, and failed to activate benzo[a]pyrene and aflatoxin B1 to mutagenic metabolites. These results provide evidence that both biochemical parameters and the overall capacity of fish liver to activate or detoxify certain mutagens can be assumed to be sensitive indicators of exposure to mixed organic pollutants in the marine environment.
- Subjects :
- Animals
Biotransformation
Cytosol enzymology
Cytosol metabolism
Glutathione metabolism
Liver enzymology
Liver metabolism
Microsomes, Liver metabolism
Mixed Function Oxygenases drug effects
Muscles chemistry
Mutagenicity Tests
Organ Size
Seawater
Carcinogens, Environmental metabolism
Fishes metabolism
Liver drug effects
Mutagens metabolism
Water Pollutants pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0027-5107
- Volume :
- 262
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Mutation research
- Publication Type :
- Academic Journal
- Accession number :
- 1705659
- Full Text :
- https://doi.org/10.1016/0165-7992(91)90119-o