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Degeneration and regeneration of ultraviolet cone photoreceptors during development in rainbow trout.
- Source :
-
The Journal of comparative neurology [J Comp Neurol] 2006 Dec 10; Vol. 499 (5), pp. 702-15. - Publication Year :
- 2006
-
Abstract
- Ultraviolet-sensitive (UVS) cones disappear from the retina of salmonid fishes during a metamorphosis that prepares them for deeper/marine waters. UVS cones subsequently reappear in the retina near sexual maturation and the return migration to natal streams. Cellular mechanisms of this UVS cone ontogeny were investigated using electroretinograms, in situ hybridization, and immunohistochemistry against opsins during and after thyroid hormone (TH) treatments of rainbow trout (Oncorhynchus mykiss). Increasing TH levels led to UVS cone degeneration. Labeling demonstrated that UVS cone degeneration occurs via programmed cell death and caspase inhibitors can inhibit this death. After the cessation of TH treatment, UVS cones regenerated in the retina. Bromodeoxyuridine (BrdU) was applied after the termination of TH treatment and was detected in the nuclei of cells expressing UVS opsin. BrdU was found in UVS cones but not other cone types. The most parsimonious explanation for the data is that UVS cones degenerated and UVS cones were regenerated from intrinsic retinal progenitor cells. Regenerating UVS cones were functionally integrated such that they were able to elicit electrical responses from second-order neurons. This is the first report of cones regenerating during natural development. Both the death and regeneration of cones in retinae represent novel mechanisms for tuning visual systems to new visual tasks or environments.
- Subjects :
- Animals
Electroretinography
Immunohistochemistry
In Situ Hybridization
Retina anatomy & histology
Retina pathology
Retinal Cone Photoreceptor Cells cytology
Retinal Cone Photoreceptor Cells drug effects
Rod Opsins metabolism
Stem Cells cytology
Stem Cells physiology
Thyroid Hormones pharmacology
Ultraviolet Rays
Metamorphosis, Biological
Oncorhynchus mykiss anatomy & histology
Oncorhynchus mykiss growth & development
Regeneration
Retinal Cone Photoreceptor Cells growth & development
Retinal Cone Photoreceptor Cells physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9967
- Volume :
- 499
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- The Journal of comparative neurology
- Publication Type :
- Academic Journal
- Accession number :
- 17048226
- Full Text :
- https://doi.org/10.1002/cne.21164