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Paenibacillus ginsengisoli sp. nov., a novel bacterium isolated from soil of a ginseng field in Pocheon Province, South Korea.
- Source :
-
Antonie van Leeuwenhoek [Antonie Van Leeuwenhoek] 2007 Feb; Vol. 91 (2), pp. 127-35. - Publication Year :
- 2007
-
Abstract
- A Gram-positive, aerobic or facultative anaerobic, motile, spore-forming bacterial strain, designated Gsoil 1638T, was isolated from a soil sample of a ginseng field in Pocheon province (South Korea), and was characterized taxonomically by using a polyphasic approach. It grew well on nutrient agar medium, utilized a fairly narrow spectrum of carbon sources and tolerated 10% NaCl. The isolate was positive for catalase and oxidase tests but negative for the degradation of macromolecules such as casein, collagen, starch, chitin, CM-cellulose, xylan and DNA. The G + C content of the genomic DNA was 50.7 mol%. The predominant isoprenoid quinone was menaquinone 7 (MK-7). The major fatty acids were anteiso-C15:0 (44%) and C16:0 (25%). Comparative 16S rRNA gene sequence analysis showed that strain Gsoil 1638T fell within the radiation of the cluster comprising Paenibacillus species and joined Paenibacillus anaericanus DSM 15890T with a bootstrap value of 100%. These two strains shared 99.5% 16S rRNA gene sequence similarity with each other. The phylogenetic distance from any other validly described species within the genus Paenibacillus was less than 96.2%. DNA-DNA relatedness value between strain Gsoil 1638T and its closest phylogenetic neighbor, Paenibacillus anaericanus, was 62%. On the basis of its phenotypic properties and phylogenetic distinctiveness, strain Gsoil 1638T (= KCTC 13931T = LMG 23406T = CCUG 52472T) was classified in the genus Paenibacillus as the type strain of a novel species, for which the name Paenibacillus ginsengisoli sp. nov. is proposed.
- Subjects :
- Aerobiosis
Anaerobiosis
Bacterial Typing Techniques
Base Composition
Carbon metabolism
Catalase analysis
DNA, Bacterial chemistry
DNA, Bacterial genetics
DNA, Ribosomal chemistry
DNA, Ribosomal genetics
Fatty Acids analysis
Genes, rRNA
Gram-Positive Endospore-Forming Bacteria chemistry
Gram-Positive Endospore-Forming Bacteria physiology
Korea
Molecular Sequence Data
Movement
Nucleic Acid Hybridization
Oxidoreductases analysis
Panax
Phylogeny
Quinones analysis
RNA, Bacterial genetics
RNA, Ribosomal, 16S genetics
Sequence Analysis, DNA
Sequence Homology, Nucleic Acid
Gram-Positive Endospore-Forming Bacteria classification
Gram-Positive Endospore-Forming Bacteria isolation & purification
Soil Microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 0003-6072
- Volume :
- 91
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Antonie van Leeuwenhoek
- Publication Type :
- Academic Journal
- Accession number :
- 17043912
- Full Text :
- https://doi.org/10.1007/s10482-006-9102-x