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5'-Triphosphate RNA is the ligand for RIG-I.
- Source :
-
Science (New York, N.Y.) [Science] 2006 Nov 10; Vol. 314 (5801), pp. 994-7. Date of Electronic Publication: 2006 Oct 12. - Publication Year :
- 2006
-
Abstract
- The structural basis for the distinction of viral RNA from abundant self RNA in the cytoplasm of virally infected cells is largely unknown. We demonstrated that the 5'-triphosphate end of RNA generated by viral polymerases is responsible for retinoic acid-inducible protein I (RIG-I)-mediated detection of RNA molecules. Detection of 5'-triphosphate RNA is abrogated by capping of the 5'-triphosphate end or by nucleoside modification of RNA, both occurring during posttranscriptional RNA processing in eukaryotes. Genomic RNA prepared from a negative-strand RNA virus and RNA prepared from virus-infected cells (but not from noninfected cells) triggered a potent interferon-alpha response in a phosphatase-sensitive manner. 5'-triphosphate RNA directly binds to RIG-I. Thus, uncapped 5'-triphosphate RNA (now termed 3pRNA) present in viruses known to be recognized by RIG-I, but absent in viruses known to be detected by MDA-5 such as the picornaviruses, serves as the molecular signature for the detection of viral infection by RIG-I.
- Subjects :
- Animals
Cell Line
Cells, Cultured
Cytosol metabolism
Cytosol virology
DEAD Box Protein 58
DNA-Directed RNA Polymerases metabolism
Humans
Interferon-alpha biosynthesis
Interferon-beta biosynthesis
Ligands
Mice
Monocytes metabolism
Oligoribonucleotides metabolism
Phosphates metabolism
Phosphorylation
RNA chemistry
RNA Caps metabolism
RNA, Double-Stranded chemistry
RNA, Double-Stranded metabolism
RNA, Viral chemistry
Rabies virus genetics
Rabies virus immunology
Rabies virus physiology
Receptors, Immunologic
Transcription, Genetic
Transfection
Viral Proteins metabolism
Virus Replication
DEAD-box RNA Helicases metabolism
RNA metabolism
RNA, Viral metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1095-9203
- Volume :
- 314
- Issue :
- 5801
- Database :
- MEDLINE
- Journal :
- Science (New York, N.Y.)
- Publication Type :
- Academic Journal
- Accession number :
- 17038590
- Full Text :
- https://doi.org/10.1126/science.1132505