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Carbonic anhydrase II gene expression in cell lines from human pancreatic adenocarcinoma.
- Source :
-
Pancreas [Pancreas] 1990 Sep; Vol. 5 (5), pp. 507-14. - Publication Year :
- 1990
-
Abstract
- Current evidence suggests that carbonic anhydrase II (CA II) is produced by pancreatic duct cells but not by pancreatic acinar or islet cells. The aim of this study was to determine whether CA II homologous RNA and CA II immunoreactive protein are produced by cell lines established from human pancreatic adenocarcinomas. A 1.7-Kb CA II homologous RNA was detected in poly(A+) RNA isolated from normal human pancreas, normal human liver, and to varying degrees in the cell lines examined. The CA II immunoreactivity corresponding to approximately 30 kD (consistent with the established molecular mass of CA II) was also detected by immunoblotting in normal human pancreas, normal human liver, and some of the cell lines. We also found that the levels of CA II homologous RNA increase in the pancreatic adenocarcinoma cell lines following treatment with the differentiating agent, retinoic acid.
- Subjects :
- Cytosol enzymology
Humans
Immunoblotting
Liver enzymology
Molecular Weight
Nucleic Acid Hybridization
Pancreas enzymology
Poly A analysis
Poly A metabolism
RNA analysis
RNA metabolism
RNA, Messenger
Tretinoin pharmacology
Tumor Cells, Cultured
Adenocarcinoma enzymology
Carbonic Anhydrases genetics
Gene Expression
Pancreatic Neoplasms enzymology
Subjects
Details
- Language :
- English
- ISSN :
- 0885-3177
- Volume :
- 5
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Pancreas
- Publication Type :
- Academic Journal
- Accession number :
- 1700414
- Full Text :
- https://doi.org/10.1097/00006676-199009000-00002