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Effect of fibroblasts on tracheal epithelial regeneration in vitro.
- Source :
-
Tissue engineering [Tissue Eng] 2006 Sep; Vol. 12 (9), pp. 2619-28. - Publication Year :
- 2006
-
Abstract
- Several artificial grafts for covering deficient trachea have been produced through tissue engineering. Recently, our group clinically used an artificial trachea made from collagen sponge for patients with noncircumferential tracheal resection. However, the slowness of epithelial regeneration on the surface of the artificial trachea was confirmed as one particular problem. In this study, we co-cultured tracheal epithelial cells with fibroblasts and examined effects of fibroblasts on epithelial regeneration in vitro. Fibroblasts activated epithelial cell proliferation and migration. In co-culture with fibroblasts, epithelial cells reconstructed pseudostratified epithelium, which was composed of ciliated, goblet, and basal cells. Furthermore, a basement membrane was reconstructed between epithelial cells and fibroblasts, and integrin beta4 was also observed there. Fibroblasts rapidly increased mucin secretion by epithelial cells. These results indicate that stimulatory effects of fibroblasts on epithelial cell migration, proliferation, and differentiation would reduce the time required for covering of epithelial cells on the defect of luminal surface and hasten regeneration of morphologically and functionally normalized epithelium involving the reconstruction of basement membrane.
- Subjects :
- Animals
Animals, Genetically Modified
Bioartificial Organs
Cell Differentiation physiology
Cell Movement physiology
Cell Proliferation
Coculture Techniques
Epithelial Cells cytology
Epithelium physiology
Fibroblasts cytology
Humans
Rats
Rats, Sprague-Dawley
Tissue Engineering methods
Trachea cytology
Trachea injuries
Epithelial Cells physiology
Fibroblasts physiology
Regeneration physiology
Trachea physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1076-3279
- Volume :
- 12
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Tissue engineering
- Publication Type :
- Academic Journal
- Accession number :
- 16995795
- Full Text :
- https://doi.org/10.1089/ten.2006.12.2619