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In vitro organogenesis and alkaloid accumulation in Datura innoxia.
- Source :
-
Zeitschrift fur Naturforschung. C, Journal of biosciences [Z Naturforsch C J Biosci] 2006 Jul-Aug; Vol. 61 (7-8), pp. 560-4. - Publication Year :
- 2006
-
Abstract
- The kinetics of tropane alkaloids accumulation in different organs such as roots, leaves, stems, flowers and seeds of Datura innoxia was investigated by GC-MS. Twenty-six tropane alkaloids were detected. The ester derivatives of tropine (3alpha-tigloyloxytropine and 3-tigloyloxy-6-hydroxytropine) are the major compounds. Undifferentiated callus were established from the stem explants of Datura innoxia using Murashige and Skoog (MS) medium supplied with 6-benzylaminopurine (BA, 1 mg l(-10) and indole-3-acetic acid (IAA, 0.5 mg l(-1)) in combination for 6 weeks. Callus differentiation was initiated by subculture onto solid MS medium, free from hormones, for more than 10 months. Initially, shoots were formed after four weeks from subculture. Further subculturing in basal MS medium without growth regulators initiated the rooting of a shooty callus after 6 weeks. Investigation of the alkaloid content of the unorganized and organized callus revealed that callus (either green or brown) yielded only trace amounts of alkaloids. On the other hand, re-differentiated shoots contained mainly scopolamine while re-differentiated roots biosynthesized hyoscyamine as the main alkaloid.
- Subjects :
- Alkaloids chemistry
Benzyl Compounds chemistry
Benzyl Compounds metabolism
Cells, Cultured
Datura cytology
Gas Chromatography-Mass Spectrometry
Germination
Indoleacetic Acids chemistry
Indoleacetic Acids metabolism
Meristem growth & development
Meristem metabolism
Plant Shoots growth & development
Plant Shoots metabolism
Plant Stems growth & development
Plant Stems metabolism
Purines chemistry
Purines metabolism
Alkaloids metabolism
Datura growth & development
Datura metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0939-5075
- Volume :
- 61
- Issue :
- 7-8
- Database :
- MEDLINE
- Journal :
- Zeitschrift fur Naturforschung. C, Journal of biosciences
- Publication Type :
- Academic Journal
- Accession number :
- 16989317
- Full Text :
- https://doi.org/10.1515/znc-2006-7-815