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Differences between tissue-associated intestinal microfloras of patients with Crohn's disease and ulcerative colitis.
- Source :
-
Journal of clinical microbiology [J Clin Microbiol] 2006 Nov; Vol. 44 (11), pp. 4136-41. Date of Electronic Publication: 2006 Sep 20. - Publication Year :
- 2006
-
Abstract
- A leading hypothesis for the role of bacteria in inflammatory bowel diseases is that an imbalance in normal gut flora is a prerequisite for inflammation. Testing this hypothesis requires comparisons between the microbiota compositions of ulcerative colitis and Crohn's disease patients and those of healthy individuals. In this study, we obtained biopsy samples from patients with Crohn's disease and ulcerative colitis and from healthy controls. Bacterial DNA was extracted from the tissue samples, amplified using universal bacterial 16S rRNA gene primers, and cloned into a plasmid vector. Insert-containing colonies were picked for high-throughput sequencing, and sequence data were analyzed, yielding species-level phylogenetic data. The clone libraries yielded 3,305 sequenced clones, representing 151 operational taxonomical units. There was no significant difference between floras from inflamed and healthy tissues from within the same individual. Proteobacteria were significantly (P = 0.0007) increased in Crohn's disease patients, as were Bacteroidetes (P < 0.0001), while Clostridia were decreased in that group (P < 0.0001) in comparison with the healthy and ulcerative colitis groups, which displayed no significant differences. Thus, the bacterial flora composition of Crohn's patients appears to be significantly altered from that of healthy controls, unlike that of ulcerative colitis patients. Imbalance in flora in Crohn's disease is probably not sufficient to cause inflammation, since microbiotas from inflamed and noninflamed tissues were of similar compositions within the same individual.
- Subjects :
- Adult
Aged
Archaea isolation & purification
Bacteroides isolation & purification
Clostridium isolation & purification
Female
Humans
Male
Middle Aged
Proteobacteria isolation & purification
Bacteria isolation & purification
Colitis, Ulcerative microbiology
Crohn Disease microbiology
Intestines microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 0095-1137
- Volume :
- 44
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Journal of clinical microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 16988016
- Full Text :
- https://doi.org/10.1128/JCM.01004-06