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Keratin subtypes in carcinomas of the uterine cervix: implications for histogenesis and differential diagnosis.
- Source :
-
Cancer research [Cancer Res] 1990 Aug 15; Vol. 50 (16), pp. 5143-52. - Publication Year :
- 1990
-
Abstract
- Normal epithelia and carcinomas of the human uterine cervix were studied by monoclonal antibodies chain specific for cytokeratins 4, 8, 10, 13, 14, 18, and 19. Most cells in 13 examined squamous carcinomas revealed a cytokeratin phenotype detected in ectocervical basal cells and endocervical subcolumnar reserve cells: 8+, 14+, 18+, 19+, 4-, 10-, 13-. We propose that these two cell types are closely related or identical and that squamous carcinoma of the cervix originates in this cell type. In more differentiated tumor cells cytokeratins 4, 10, and 13, which are present in suprabasal layers of the normal ectocervical epithelium, were coexpressed with basal cell cytokeratins. Thus, contrary to previous beliefs, all cytokeratins detected in carcinomas were also present in normal epithelium of uterine cervix. The cytokeratin profile of cervical adenocarcinomas corresponded to that of columnar endocervical cells (8+, 18+, 19+), although two of the three adenocarcinomas also expressed cytokeratin 4, which in the normal endocervix was detected in scattered single columnar cells only. The new monoclonal antibody DE-K14, specific for cytokeratin 14, proved a specific marker of subcolumnar reserve cells in the endocervix. It was also the only one that reacted with all cervical squamous carcinomas but with none of the cervical adenocarcinomas and, as such, has a potential value for pathological differential diagnosis of cervical tumors.
- Subjects :
- Adenocarcinoma analysis
Adenocarcinoma pathology
Antibodies, Monoclonal
Carcinoma, Squamous Cell analysis
Carcinoma, Squamous Cell pathology
Cervix Uteri analysis
Electrophoresis, Gel, Two-Dimensional
Epithelial Cells
Epithelium analysis
Female
Fluorescent Antibody Technique
Humans
Immunoblotting
Keratins immunology
Keratins isolation & purification
Reference Values
Uterine Cervical Neoplasms analysis
Cervix Uteri cytology
Keratins analysis
Uterine Cervical Neoplasms pathology
Subjects
Details
- Language :
- English
- ISSN :
- 0008-5472
- Volume :
- 50
- Issue :
- 16
- Database :
- MEDLINE
- Journal :
- Cancer research
- Publication Type :
- Academic Journal
- Accession number :
- 1696167