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Immunohistochemical characterization of rat central and peripheral nerve tumors induced by ethylnitrosourea.
- Source :
-
Toxicologic pathology [Toxicol Pathol] 1990; Vol. 18 (1 Pt 1), pp. 18-23. - Publication Year :
- 1990
-
Abstract
- Ethylnitrosourea-induced central and peripheral nerve tumors in Sprague-Dawley rats were tested for GFAP (Glial Fibrillary Acidic Protein), S-100 protein, NSE (Neuron Specific Enolase) and Anti-Leu 7 (HNK-1) immunoreactivity utilizing the ABC method (avidin-biotin-complex) for GFAP, S-100 protein and NSE, and the PAP method (peroxidase-antiperoxidase) for Anti-Leu 7. Peripheral nerve neurinomas were consistently positive for S-100 protein and consistently negative for GFAP and Anti-Leu 7. Neurinomas would occasionally exhibit positive staining for NSE (2 of 55 tumors). The staining intensity for S-100 protein varied from strongly positive in differentiated neurinomas to weakly positive in anaplastic tumors. Neoplastic and reactive astrocytes exhibited positive staining for both S-100 protein and GFAP. Variation in the GFAP staining intensity of glial tumors correlated with the degree of differentiation as anaplastic tumors did not stain with the same intensity as their more differentiated counterparts. Oligodendrogliomas exhibited occasional immunoreactivity to S-100 protein (3 of 36 tumors). NSE reactivity in oligodendrogliomas was rarely observed (1 tumor in 36) and immunoreactivity against GFAP or Anti-Leu 7 was consistently absent. Anti-Leu 7 and NSE proved to be of little value in the classification of ENU-induced neural tumors.
- Subjects :
- Animals
Antigens, Differentiation metabolism
Astrocytoma chemically induced
Astrocytoma metabolism
Astrocytoma pathology
CD57 Antigens
Central Nervous System drug effects
Central Nervous System metabolism
Central Nervous System pathology
Ependymoma chemically induced
Ependymoma metabolism
Ependymoma pathology
Glial Fibrillary Acidic Protein metabolism
Glioma chemically induced
Glioma metabolism
Glioma pathology
Immunohistochemistry
Meningioma chemically induced
Meningioma metabolism
Meningioma pathology
Nervous System Neoplasms metabolism
Nervous System Neoplasms pathology
Neurilemmoma chemically induced
Neurilemmoma metabolism
Neurilemmoma pathology
Oligodendroglioma chemically induced
Oligodendroglioma metabolism
Oligodendroglioma pathology
Peripheral Nervous System Neoplasms metabolism
Peripheral Nervous System Neoplasms pathology
Phosphopyruvate Hydratase metabolism
Rats
Rats, Inbred Strains
S100 Proteins metabolism
Ethylnitrosourea toxicity
Nervous System Neoplasms chemically induced
Peripheral Nervous System Neoplasms chemically induced
Subjects
Details
- Language :
- English
- ISSN :
- 0192-6233
- Volume :
- 18
- Issue :
- 1 Pt 1
- Database :
- MEDLINE
- Journal :
- Toxicologic pathology
- Publication Type :
- Academic Journal
- Accession number :
- 1694597
- Full Text :
- https://doi.org/10.1177/019262339001800103