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The chloroplast lumen and stromal proteomes of Arabidopsis thaliana show differential sensitivity to short- and long-term exposure to low temperature.
- Source :
-
The Plant journal : for cell and molecular biology [Plant J] 2006 Sep; Vol. 47 (5), pp. 720-34. - Publication Year :
- 2006
-
Abstract
- Cold acclimation and over-wintering by herbaceous plants are energetically expensive and are dependent on functional plastid metabolism. To understand how the stroma and the lumen proteomes adapt to low temperatures, we have taken a proteomic approach (difference gel electrophoresis) to identify proteins that changed in abundance in Arabidopsis chloroplasts during cold shock (1 day), and short- (10 days) and long-term (40 days) acclimation to 5 degrees C. We show that cold shock (1 day) results in minimal change in the plastid proteomes, while short-term (10 days) acclimation results in major changes in the stromal but few changes in the lumen proteome. Long-term acclimation (40 days) results in modulation of the proteomes of both compartments, with new proteins appearing in the lumen and further modulations in protein abundance occurring in the stroma. We identify 43 differentially displayed proteins that participate in photosynthesis, other plastid metabolic functions, hormone biosynthesis and stress sensing and signal transduction. These findings not only provide new insights into the cold response and acclimation of Arabidopsis, but also demonstrate the importance of studying changes in protein abundance within the relevant cellular compartment.
- Subjects :
- Acclimatization
Arabidopsis genetics
Arabidopsis physiology
Arabidopsis Proteins classification
Arabidopsis Proteins genetics
Chloroplasts physiology
Electrophoresis, Gel, Two-Dimensional
Gene Expression Profiling
Photosynthesis
Proteomics
Signal Transduction
Time Factors
Arabidopsis metabolism
Arabidopsis Proteins metabolism
Chloroplasts metabolism
Cold Temperature
Subjects
Details
- Language :
- English
- ISSN :
- 0960-7412
- Volume :
- 47
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- The Plant journal : for cell and molecular biology
- Publication Type :
- Academic Journal
- Accession number :
- 16923014
- Full Text :
- https://doi.org/10.1111/j.1365-313X.2006.02821.x