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Bacteriophage T4 gene 32 protein shares antigen determinants with reconstituted HeLa hnRNP proteins in western blots.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 1990 Mar 30; Vol. 167 (3), pp. 1196-9. - Publication Year :
- 1990
-
Abstract
- A polyclonal antiserum against purified bacteriophage T4 gene 32 protein was raised in rabbits. In Western blots it detected a number of SDS-PAGE separated nuclear and ribosomal proteins of HeLa cells. Using a renaturing blotting system, however, larger hnRNP proteins ranging between 66.000 and 82.000 Da preferentially reacted with this antiserum. In addition hnRNP group A core proteins were detected to a minor extent. Nucleic acid binding proteins like histones or ribosomal proteins were not stained by this antibody after renaturation.
- Subjects :
- Blotting, Western
DNA-Binding Proteins isolation & purification
Escherichia coli genetics
HeLa Cells analysis
Heterogeneous-Nuclear Ribonucleoproteins
Humans
Immune Sera
Molecular Weight
Ribonucleoproteins isolation & purification
Viral Proteins isolation & purification
DNA-Binding Proteins immunology
Epitopes analysis
Ribonucleoproteins immunology
T-Phages genetics
Viral Proteins immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0006-291X
- Volume :
- 167
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 1690995
- Full Text :
- https://doi.org/10.1016/0006-291x(90)90650-c