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Modulation of microvascular growth and morphogenesis by reconstituted basement membrane gel in three-dimensional cultures of rat aorta: a comparative study of angiogenesis in matrigel, collagen, fibrin, and plasma clot.
- Source :
-
In vitro cellular & developmental biology : journal of the Tissue Culture Association [In Vitro Cell Dev Biol] 1990 Feb; Vol. 26 (2), pp. 119-28. - Publication Year :
- 1990
-
Abstract
- Rings of rat aorta cultured in Matrigel, a reconstituted gel composed of basement membrane molecules, gave rise to three-dimensional networks composed of solid cellular cords and occasional microvessels with slitlike lumina. Immunohistochemical and ultrastructural studies showed that the solid cords were composed of endothelial sprouts surrounded by nonendothelial mesenchymal cells. The angiogenic response of the aortic rings in Matrigel was compared to that obtained in interstitial collagen, fibrin, or plasma clot. Morphometric analysis demonstrated that the mean luminal area of the microvascular sprouts and channels was significantly smaller in Matrigel than in collagen, fibrin, or plasma clot. The percentage of patent microvessels in Matrigel was also markedly reduced. Autoradiographic studies of 3H-thymidine-labeled cultures showed reduced DNA synthesis by developing microvessels in Matrigel. The overall number of solid endothelial cords and microvessels was lower in Matrigel than in fibrin or plasma clot. A mixed cell population isolated from Matrigel cultures formed a monolayer in collagen or fibrin-coated dishes but rapidly reorganized into a polygonal network when plated on Matrigel. The observation that gels composed of basement membrane molecules modulate the canalization, proliferation, and organization into networks of vasoformative endothelial cells in three-dimensional cultures supports the hypothesis that the basement membrane is a potent regulator of microvascular growth and morphogenesis.
- Subjects :
- Animals
Aorta metabolism
Aorta physiology
Aorta ultrastructure
Autoradiography
Basement Membrane analysis
Basement Membrane physiology
Cell Division
Cells, Cultured
Collagen pharmacology
Drug Combinations pharmacology
Endothelium, Vascular cytology
Endothelium, Vascular physiology
Endothelium, Vascular ultrastructure
Fibrin pharmacology
Gels
Immunohistochemistry
Laminin pharmacology
Male
Microscopy, Electron
Morphogenesis physiology
Neovascularization, Pathologic pathology
Plasma physiology
Proteoglycans pharmacology
Rats
Rats, Inbred F344
von Willebrand Factor metabolism
Aorta cytology
Neovascularization, Pathologic physiopathology
Subjects
Details
- Language :
- English
- ISSN :
- 0883-8364
- Volume :
- 26
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- In vitro cellular & developmental biology : journal of the Tissue Culture Association
- Publication Type :
- Academic Journal
- Accession number :
- 1690206
- Full Text :
- https://doi.org/10.1007/BF02624102