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Transcriptomic analysis of extensive changes in metabolic regulation in Kluyveromyces lactis strains.
- Source :
-
Eukaryotic cell [Eukaryot Cell] 2006 Aug; Vol. 5 (8), pp. 1360-70. - Publication Year :
- 2006
-
Abstract
- Genome-wide analysis of transcriptional regulation is generally carried out on well-characterized reference laboratory strains; hence, the characteristics of industrial isolates are therefore overlooked. In a previous study on the major cheese yeast Kluyveromyces lactis, we have shown that the reference strain and an industrial strain used in cheese making display a differential gene expression when grown on a single carbon source. Here, we have used more controlled conditions, i.e., growth in a fermentor with pH and oxygen maintained constant, to study how these two isolates grown in glucose reacted to an addition of lactose. The observed differences between sugar consumption and the production of various metabolites, ethanol, acetate, and glycerol, correlated with the response were monitored by the analysis of the expression of 482 genes. Extensive differences in gene expression between the strains were revealed in sugar transport, glucose repression, ethanol metabolism, and amino acid import. These differences were partly due to repression by glucose and another, yet-unknown regulation mechanism. Our results bring to light a new type of K. lactis strain with respect to hexose transport gene content and repression by glucose. We found that a combination of point mutations and variation in gene regulation generates a biodiversity within the K. lactis species that was not anticipated. In contrast to S. cerevisiae, in which there is a massive increase in the number of sugar transporter and fermentation genes, in K. lactis, interstrain diversity in adaptation to a changing environment is based on small changes at the level of key genes and cell growth control.
- Subjects :
- Acetates analysis
Acetates metabolism
Ethanol analysis
Ethanol metabolism
Gene Expression Profiling
Glucose metabolism
Glucose pharmacology
Glucose Transport Proteins, Facilitative metabolism
Glycerol analysis
Glycerol metabolism
Kluyveromyces classification
Lactose metabolism
Membrane Transport Proteins drug effects
Membrane Transport Proteins genetics
Oligonucleotide Array Sequence Analysis
Pentose Phosphate Pathway drug effects
Pentose Phosphate Pathway genetics
Adaptation, Physiological
Gene Expression Regulation, Fungal
Kluyveromyces genetics
Kluyveromyces metabolism
Transcription, Genetic
Subjects
Details
- Language :
- English
- ISSN :
- 1535-9778
- Volume :
- 5
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Eukaryotic cell
- Publication Type :
- Academic Journal
- Accession number :
- 16896219
- Full Text :
- https://doi.org/10.1128/EC.00087-06