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Baculovirus expression of BmAChE3, a cDNA encoding an acetylcholinesterase of Boophilus microplus (Acari: Ixodidae).
- Source :
-
Journal of medical entomology [J Med Entomol] 2006 Jul; Vol. 43 (4), pp. 707-12. - Publication Year :
- 2006
-
Abstract
- The complete cDNA sequence encoding a Boophilus microplus (Canestrini) (Acari: Ixodidae) acetylcholinesterase (AChE3) was expressed in the baculovirus system. The recombinant AChE3 protein (rBmAChE3) was secreted as a soluble form into the cell culture medium and was identified as a functional AChE by substrate specificity and by inhibition with the AChE-specific inhibitors eserine sulfate and BW284c51. Inhibition kinetics of rBmAChE3, in the presence of the organophosphate paraoxon, revealed sensitivity comparable with that of adult, organophosphate-susceptible neural AChE. To our knowledge, this is the first report of the cloning and successful expression of a functional ixodid AChE.
- Subjects :
- Acetylcholinesterase chemistry
Acetylcholinesterase genetics
Acetylcholinesterase metabolism
Animals
Arachnid Vectors enzymology
Arachnid Vectors genetics
Arachnid Vectors physiology
Baculoviridae genetics
Benzenaminium, 4,4'-(3-oxo-1,5-pentanediyl)bis(N,N-dimethyl-N-2-propenyl-), Dibromide pharmacology
Cattle
Cholinesterase Inhibitors pharmacology
Cloning, Molecular methods
DNA Primers chemistry
DNA, Complementary physiology
Insecticide Resistance genetics
Ixodidae genetics
Paraoxon pharmacology
Physostigmine pharmacology
Transfection
Acetylcholinesterase biosynthesis
Gene Expression Regulation, Enzymologic physiology
Insecticide Resistance physiology
Ixodidae enzymology
Ixodidae physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-2585
- Volume :
- 43
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Journal of medical entomology
- Publication Type :
- Academic Journal
- Accession number :
- 16892628
- Full Text :
- https://doi.org/10.1603/0022-2585(2006)43[707:beobac]2.0.co;2