[Developing of a new multicolor-fluorescent labeled STR amplification kit].

Authors :: Li L
Chen GH
Li CT
Liu Y
Lin Y
Que TZ
Zhao ZM
Wang YX
Source :: Fa yi xue za zhi [Fa Yi Xue Za Zhi] 2006 Apr; Vol. 22 (2), pp. 111-6.
Publication Year :: 2006
Abstract: Objective: To develop a PCR-based STR system for genotyping of 18 loci (Amelogenin, D3S1358, vWA, FGA, D8S1179, D21S11, D18S51, D5S818, D13S317, D16S539, TH01, TPOX, CSF1PO, D7S820, D2S1338, D19S433, D12S391 and D19S253).<br />Methods: By using primers labeled with four color fluorescent (FAM, HEX, TAMRA and ROX), two multiplex amplification reaction systems were developed to genotype Amelogenin and 17 STR loci.<br />Results: Amelogenin and these 17 STR loci were genotyped successfully in different kinds of biological samples by the kit.<br />Conclusion: The STR amplification kit developed in our study gives a new approach to genotype these 18 loci in a efficient, steady and reliable way.