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Lactoferricin-related peptides with inhibitory effects on ACE-dependent vasoconstriction.
- Source :
-
Journal of agricultural and food chemistry [J Agric Food Chem] 2006 Jul 26; Vol. 54 (15), pp. 5323-9. - Publication Year :
- 2006
-
Abstract
- A selection of lactoferricin B (LfcinB)-related peptides with an angiotensin I-converting enzyme (ACE) inhibitory effect have been examined using in vitro and ex vivo functional assays. Peptides that were analyzed included a set of sequence-related antimicrobial hexapeptides previously reported and two representative LfcinB-derived peptides. In vitro assays using hippuryl-L-histidyl-L-leucine (HHL) and angiotensin I as substrates allowed us to select two hexapeptides, PACEI32 (Ac-RKWHFW-NH2) and PACEI34 (Ac-RKWLFW-NH2), and also a LfcinB-derived peptide, LfcinB17-31 (Ac-FKCRRWQWRMKKLGA-NH2). Ex vivo functional assays using rabbit carotid arterial segments showed PACEI32 (both D- and L-enantiomers) and LfcinB17-31 have inhibitory effects on ACE-dependent angiotensin I-induced contraction. None of the peptides exhibited in vitro ACE inhibitory activity using bradykinin as the substrate. In conclusion, three bioactive lactoferricin-related peptides exhibit inhibitory effects on both ACE activity and ACE-dependent vasoconstriction with potential to modulate hypertension that deserves further investigation.
- Subjects :
- Angiotensin I pharmacology
Animals
Carotid Artery, Common drug effects
Carotid Artery, Common physiology
In Vitro Techniques
Lactoferrin chemistry
Male
Peptides chemistry
Peptidyl-Dipeptidase A physiology
Rabbits
Angiotensin-Converting Enzyme Inhibitors pharmacology
Lactoferrin pharmacology
Peptides pharmacology
Vasoconstriction drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 0021-8561
- Volume :
- 54
- Issue :
- 15
- Database :
- MEDLINE
- Journal :
- Journal of agricultural and food chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 16848512
- Full Text :
- https://doi.org/10.1021/jf060482j