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Structural and inhibitory properties of a plant proteinase inhibitor containing the RGD motif.
- Source :
-
International journal of biological macromolecules [Int J Biol Macromol] 2006 Dec 15; Vol. 40 (1), pp. 22-9. Date of Electronic Publication: 2006 May 26. - Publication Year :
- 2006
-
Abstract
- Purified from Bauhinia rufa seeds, BrTI is a Kunitz proteinase inhibitor that contains the RGD sequence. BrTI inhibits trypsin (K(iapp) 2.9 nM) and human plasma kallikrein (K(iapp) 14.0 nM) but not other related enzymes. The synthetic peptide YLEPVARGDGGLA-NH(2) (70 microM) inhibited the adhesion to fibronectin of B16F10 (high-metastatic B16 murine mouse melanoma cell line) and of Tm5 (murine melanoma cell lines derived from a non-tumorigenic lineage of pigmented murine melanocytes, melan-a). YLEPVARGEGGLA-NH(2) in which Asp(9) was changed into Glu does not affect the cell attachment. Moreover, this peptide was functional only when the sequence present in the native protein was preserved, since YLIPVARGDGGLA-NH(2) in which Glu(3) was changed into Ile does not interfere with B16F10 and was less effective on Tm5 cell line adhesion. Neither YLEPVARGDGGLA-NH(2), YLIPVARGDGGLA-NH(2) or YLEPVARGEGGLA-NH(2) inhibit the interaction of RAEC (endothelial cell line from rabbit aorta) with fibronectin.
- Subjects :
- Amino Acid Sequence
Animals
Bauhinia genetics
Cell Adhesion drug effects
Cell Line, Tumor
Endothelial Cells cytology
Endothelial Cells drug effects
Humans
In Vitro Techniques
Kallikreins antagonists & inhibitors
Melanoma, Experimental drug therapy
Mice
Molecular Sequence Data
Oligopeptides
Peptides genetics
Plant Proteins genetics
Rabbits
Sequence Homology, Amino Acid
Bauhinia chemistry
Peptides chemistry
Peptides pharmacology
Plant Proteins chemistry
Plant Proteins pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0141-8130
- Volume :
- 40
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- International journal of biological macromolecules
- Publication Type :
- Academic Journal
- Accession number :
- 16846639
- Full Text :
- https://doi.org/10.1016/j.ijbiomac.2006.05.008