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HIV infection of mononuclear cells is calcium-dependent.
HIV infection of mononuclear cells is calcium-dependent.
- Source :
-
Virus research [Virus Res] 2006 Dec; Vol. 122 (1-2), pp. 183-8. Date of Electronic Publication: 2006 Jul 13. - Publication Year :
- 2006
-
Abstract
- Strategies that prevent initial HIV infection of cells are greatly needed. In this study, we determined the requirement of divalent cations for HIV infection of and attachment to peripheral blood mononuclear cells (PBMC), which contain several types of HIV-infectable cells-CD4(+) T cells, monocytes and dendritic cells. EDTA, added only during PBMC exposure to HIV, reduced infection by an average of 92%. The reduction of infection by EDTA was accompanied by a reduction in HIV binding to PBMC; R5, X4 and dual-tropic HIV binding to PBMC were inhibited by >85%. EGTA similarly reduced HIV binding to PBMC, while addition of Ca(2+) or Mn(2+), but not Mg(2+), fully restored binding. Virus attachment was inhibited in a dose-dependent manner by trypsin treatment of PBMC, indicating protein involvement in HIV binding. In contrast, mannan or soluble ICAM-1 did not inhibit HIV binding to PBMC. These data indicate that a Ca(2+)-dependent cell-surface protein(s) is responsible for the majority of HIV attachment to and infection of PBMC. Further studies of this are likely to reveal novel strategies to prevent infection of PBMC.
- Subjects :
- Antiviral Agents pharmacology
Cells, Cultured
Edetic Acid pharmacology
HIV Core Protein p24 biosynthesis
Humans
Intercellular Adhesion Molecule-1 pharmacology
Magnesium pharmacology
Manganese pharmacology
Mannans pharmacology
Trypsin pharmacology
Calcium metabolism
HIV physiology
Leukocytes, Mononuclear virology
Virus Attachment
Virus Internalization
Subjects
Details
- Language :
- English
- ISSN :
- 0168-1702
- Volume :
- 122
- Issue :
- 1-2
- Database :
- MEDLINE
- Journal :
- Virus research
- Publication Type :
- Academic Journal
- Accession number :
- 16842879
- Full Text :
- https://doi.org/10.1016/j.virusres.2006.06.006