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Inflammation, heat shock proteins and periodontal pathogens in atherosclerosis: an immunohistologic study.
- Source :
-
Oral microbiology and immunology [Oral Microbiol Immunol] 2006 Aug; Vol. 21 (4), pp. 206-11. - Publication Year :
- 2006
-
Abstract
- Background: Inflammation is a significant component of atherosclerosis lesions. Bacteria, including periodontopathogens, have been demonstrated in atherosclerotic plaques and cross-reactivity of the immune response to bacterial GroEL with human heat shock protein 60 has been suggested as a link between infections and atherosclerosis.<br />Methods: In this study, the nature of the inflammatory infiltrate and the presence of human heat shock protein 60 and GroEL were examined in 31 carotid endarterectomy specimens. Additionally, monoclonal antibodies were used to detect the presence of six bacteria, including those implicated in periodontal disease.<br />Results: The inflammatory cell infiltrate of the lesions was dominated by CD14(+) macrophages and CD4(+) T cells. Most cells of the infiltrate as well as the endothelium were HLA-DR(+), indicating activation; however, there was an absence of CD25 expression, demonstrating that the activated T cells were not proliferating. Few CD1a(+) and CD83(+) cells were noted. Human heat shock protein 60 expression was evident on endothelial cells and cells with the appearance of smooth muscle cells and lymphocytes. GroEL and bacteria were detected within intimal cells. Chlamydia pneumoniae, Porphyromonas gingivalis, Fusobacterium nucleatum, Tannerella forsythia, Prevotella intermedia, and Actinobacillus actinomycetemcomitans were found in 21%, 52%, 34%, 34%, 41%, and 17% of arteries, respectively.<br />Conclusion: These results give evidence for a specific immune response associated with atherosclerosis. Whether bacteria initiate the observed inflammation in atherosclerotic lesions is not clear; however, the present study shows that maintenance of inflammation may be enhanced by the presence of periodontopathic bacteria.
- Subjects :
- Aggregatibacter actinomycetemcomitans isolation & purification
Antigens, CD analysis
Antigens, CD1 analysis
Atherosclerosis immunology
Atherosclerosis microbiology
Bacteroides isolation & purification
CD4-Positive T-Lymphocytes pathology
Chlamydophila pneumoniae isolation & purification
Endarterectomy, Carotid
Endothelial Cells pathology
Endothelium, Vascular immunology
Endothelium, Vascular pathology
Fusobacterium nucleatum isolation & purification
HLA-DR Antigens analysis
Humans
Immunoglobulins analysis
Immunohistochemistry
Inflammation pathology
Lipopolysaccharide Receptors analysis
Lymphocyte Activation immunology
Macrophages pathology
Membrane Glycoproteins analysis
Muscle, Smooth, Vascular pathology
Porphyromonas gingivalis isolation & purification
Prevotella intermedia isolation & purification
Tunica Intima microbiology
CD83 Antigen
Atherosclerosis pathology
Chaperonin 60 analysis
Periodontal Diseases microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 0902-0055
- Volume :
- 21
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Oral microbiology and immunology
- Publication Type :
- Academic Journal
- Accession number :
- 16842503
- Full Text :
- https://doi.org/10.1111/j.1399-302X.2006.00276.x