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DNA-binding properties of the antibody specific for the Dewar photoproduct of thymidylyl-(3-5')-thymidine.
- Source :
-
Nucleosides, nucleotides & nucleic acids [Nucleosides Nucleotides Nucleic Acids] 2006; Vol. 25 (4-6), pp. 667-79. - Publication Year :
- 2006
-
Abstract
- A monoclonal antibody (DEM-1) specific for the Dewar photoproduct is used for detection and quantification of photolesions in DNA. To help understand the molecular recognition of damaged DNA by the antibody protein, we have cloned and sequenced the variable region genes of DEM-1. We have also prepared Fab fragments of DEM-1 (DEM1Fab), and synthesized two kinds of 3'-biotinylated oligonucleotides of different lengths containing a central Dewar photoproduct of TpT to analyze the effects of the antigen size on the binding rates by means of surface plasmon resonance (SPR). Results obtained from SPR analyses suggest that DEM1Fab may recognize tetranucleotide unit as the epitope.
- Subjects :
- Amino Acid Sequence
Antibody Specificity
DNA radiation effects
Immunoglobulin Fab Fragments immunology
Molecular Sequence Data
Molecular Structure
Photochemistry
Thymidine analogs & derivatives
Thymidine chemistry
Antibodies immunology
DNA chemistry
DNA immunology
Thymidine immunology
Thymidine radiation effects
Subjects
Details
- Language :
- English
- ISSN :
- 1525-7770
- Volume :
- 25
- Issue :
- 4-6
- Database :
- MEDLINE
- Journal :
- Nucleosides, nucleotides & nucleic acids
- Publication Type :
- Academic Journal
- Accession number :
- 16838854
- Full Text :
- https://doi.org/10.1080/15257770600686469