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Protein kinase C delta is essential to maintain CIITA gene expression in B cells.
- Source :
-
Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 2006 Jul 15; Vol. 177 (2), pp. 950-6. - Publication Year :
- 2006
-
Abstract
- Expression of MHC class II genes requires CIITA. Although the transactivation function of CIITA is well characterized, the signaling events that regulate CIITA expression are less understood. In this study, we report that CIITA expression in B cells depends on protein kinase Cdelta (PKCdelta). PKCdelta controls CIITA gene transcription mainly via modulating CREB recruitment to the CIITA promoter without affecting CIITA mRNA stability. Inhibition of PKCdelta by a pharmacological inhibitor or knocking down of endogenous PKCdelta expression by small interfering RNA reduced CREB binding to the CIITA promoter. The decrease of CIITA gene expression in the presence of the PKCdelta inhibitor was prevented by ectopically expressing a constitutively active form of CREB. In addition, histone acetylation of the CIITA promoter is regulated by PKCdelta since the PKCdelta inhibitor treatment or PKCdelta small interfering RNA resulted in decreased histone acetylation. Taken together, our study reveals that PKCdelta is an important signaling molecule necessary to maintain CIITA and MHC class II expression in B cells.
- Subjects :
- B-Lymphocytes enzymology
Cell Line
Cell Line, Tumor
Cells, Cultured
Cyclic AMP Response Element-Binding Protein metabolism
Cyclic AMP Response Element-Binding Protein physiology
Down-Regulation immunology
Humans
Nuclear Proteins antagonists & inhibitors
Nuclear Proteins metabolism
Phosphorylation
Promoter Regions, Genetic immunology
Protein Kinase C-delta antagonists & inhibitors
Protein Kinase Inhibitors pharmacology
RNA Stability immunology
RNA, Messenger antagonists & inhibitors
RNA, Messenger biosynthesis
Trans-Activators antagonists & inhibitors
Trans-Activators metabolism
B-Lymphocytes immunology
B-Lymphocytes metabolism
Nuclear Proteins biosynthesis
Nuclear Proteins genetics
Protein Kinase C-delta physiology
Trans-Activators biosynthesis
Trans-Activators genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1767
- Volume :
- 177
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Journal of immunology (Baltimore, Md. : 1950)
- Publication Type :
- Academic Journal
- Accession number :
- 16818750
- Full Text :
- https://doi.org/10.4049/jimmunol.177.2.950