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Processing of TCP pilin by TcpJ typifies a common step intrinsic to a newly recognized pathway of extracellular protein secretion by gram-negative bacteria.
- Source :
-
Genes & development [Genes Dev] 1991 Oct; Vol. 5 (10), pp. 1834-46. - Publication Year :
- 1991
-
Abstract
- Biogenesis of the Vibrio cholerae toxin-coregulated pilus (TCP) requires the activities of at least seven accessory proteins. We demonstrate that a portion of this pathway involves a novel processing step in which a hydrophilic leader peptide is proteolytically removed from TcpA by the gene product characterized in this report, TcpJ, to yield the mature, export-competent form of the pilin. Cleavage of the pilin leader peptide is independent of known signal peptidases as demonstrated by pilin-processing profiles in Escherichia coli strains conditionally defective for production of leader peptidase or grown in the presence of the antibiotic globomycin. Additionally, pilin cleavage did not rely on the SecA protein, as evidenced by TcpA processing in azide-treated cells. These results suggest that TcpJ is representative of a new class of proteins involved in SecA-independent proteolytic cleavage of a set of atypical leader peptides during extracellular export.
- Subjects :
- Amino Acid Sequence
Base Sequence
Blotting, Southern
Cloning, Molecular
DNA genetics
DNA isolation & purification
Endopeptidases metabolism
Escherichia coli genetics
Genetic Complementation Test
Models, Structural
Molecular Sequence Data
Mutagenesis, Insertional
Protein Conformation
Protein Sorting Signals metabolism
Restriction Mapping
Sequence Homology, Nucleic Acid
Vibrio cholerae ultrastructure
Fimbriae, Bacterial metabolism
Genes, Bacterial
Membrane Proteins
Serine Endopeptidases
Vibrio cholerae genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0890-9369
- Volume :
- 5
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Genes & development
- Publication Type :
- Academic Journal
- Accession number :
- 1680773
- Full Text :
- https://doi.org/10.1101/gad.5.10.1834