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Sucrose octasulfate stimulates gastric somatostatin release.
- Source :
-
The American journal of medicine [Am J Med] 1991 Aug 08; Vol. 91 (2A), pp. 52S-57S. - Publication Year :
- 1991
-
Abstract
- To explore the mechanisms of the effects of sucralfate on the stomach, we investigated the action of sucrose octasulfate (SOS), a constituent of sucralfate, on the function of canine gastric parietal cells and somatostatin cells and in the isolated perfused intact rat stomach. Somatostatin cells from the canine gastric fundus were isolated by EDTA-collagenase dispersion and counterflow elutriation, and somatostatin-like immunoreactivity (SLI) release in response to SOS was measured by radioimmunoassay. Similar methods were used to isolate gastric parietal cells, in which gastric acid secretion was measured by uptake of a radiolabeled weak base, [14C]aminopyrine. SLI release by the intact rat stomach was examined in an isolated vascularly perfused rat stomach model. SOS, either alone or co-administered with epinephrine or gastrin heptadecapeptide (G17), dose-dependently stimulated SLI release by isolated canine fundic D-cells. At the highest doses, SOS potentiated the effect of epinephrine but not G17. Similarly, SOS potentiated the stimulating effect of dibutyryl cyclic adenosine 3',5'-monophosphate (DBcAMP), but not 12-O-tetradecanoylphorbol 13-acetate (TPA). The effect of SOS on SLI release could be inhibited by octreotide, a somatostatin analogue. SOS did not alter acid secretion by cultured canine parietal cells either in the basal state or when coadministered with acid secretagogues. In isolated perfused rat stomach studies, SOS produced a significant (60% greater than basal) increase in SLI secretion. There was a similar effect when SOS was perfused against a background of isoproterenol. SOS stimulates SLI release from gastric somatostatin cells and from the isolated perfused stomach but has no direct effect on gastric parietal cells. These actions of SOS may mediate in part the apparent ability of sucralfate to enhance gastric mucosal defense.
- Subjects :
- Aminopyrine pharmacokinetics
Animals
Bucladesine pharmacology
Dose-Response Relationship, Drug
Drug Synergism
Epinephrine pharmacology
Gastric Fundus chemistry
Gastric Fundus drug effects
Gastric Fundus metabolism
Gastrins pharmacology
Isoproterenol pharmacology
Male
Octreotide pharmacology
Parietal Cells, Gastric chemistry
Parietal Cells, Gastric metabolism
Peptides chemistry
Radioimmunoassay
Rats
Rats, Inbred Strains
Somatostatin chemistry
Sucrose pharmacology
Tetradecanoylphorbol Acetate pharmacology
Parietal Cells, Gastric drug effects
Peptides metabolism
Somatostatin metabolism
Sucrose analogs & derivatives
Subjects
Details
- Language :
- English
- ISSN :
- 0002-9343
- Volume :
- 91
- Issue :
- 2A
- Database :
- MEDLINE
- Journal :
- The American journal of medicine
- Publication Type :
- Academic Journal
- Accession number :
- 1679296
- Full Text :
- https://doi.org/10.1016/0002-9343(91)90451-3