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Synthesis of mixed sequence borane phosphonate DNA.
- Source :
-
Journal of the American Chemical Society [J Am Chem Soc] 2006 Jun 28; Vol. 128 (25), pp. 8138-9. - Publication Year :
- 2006
-
Abstract
- A novel solid-phase phosphoramidite-based method has been developed for the synthesis of borane phosphonate DNA. Keys to this new approach are replacement of the common 5'-dimethoxytrityl blocking group with a 5'-silyl ether and the use of new protecting groups on the bases (adenine, N6-dimethoxytrityl; cytosine, N4-trimethoxytrityl; guanine, N2-[9-fluorenylmethoxycarbonyl]; thymine, N3-anisoyl). Because of these developments, it is now possible for the first time to synthesize oligodeoxynucleotides having any combination of the four 2'-deoxynucleosides and both phosphate and borane phosphonate internucleotide linkages (including oligomers having exclusively borane phosphonate linkages).
Details
- Language :
- English
- ISSN :
- 0002-7863
- Volume :
- 128
- Issue :
- 25
- Database :
- MEDLINE
- Journal :
- Journal of the American Chemical Society
- Publication Type :
- Academic Journal
- Accession number :
- 16787069
- Full Text :
- https://doi.org/10.1021/ja061757e