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G protein-coupled receptor 83 overexpression in naive CD4+CD25- T cells leads to the induction of Foxp3+ regulatory T cells in vivo.
- Source :
-
Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 2006 Jul 01; Vol. 177 (1), pp. 209-15. - Publication Year :
- 2006
-
Abstract
- Foxp3 functions as a lineage specification factor for the development of naturally occurring thymus-derived CD4+CD25+ regulatory T (Treg) cells. Recent evidence suggests that naive Foxp3-CD4+CD25- T cells can be converted in the periphery into Foxp3+ Treg cells. In this study, we have identified the G protein-coupled receptor (GPR)83 to be selectively up-regulated by CD4+CD25+ Treg cells of both murine and human origin in contrast to naive CD4+CD25- or recently activated T cells. Furthermore, GPR83 was induced upon overexpression of Foxp3 in naive CD4+CD25- T cells. Transduction of naive CD4+CD25- T cells with GPR83-encoding retroviruses did not confer in vitro suppressive activity. Nevertheless, GPR83-transduced T cells were able to inhibit the effector phase of a severe contact hypersensitivity reaction of the skin, indicating that GPR83 itself or GPR83-mediated signals conferred suppressive activity to conventional CD4+ T cells in vivo. Most strikingly, this in vivo acquisition of suppressive activity was associated with the induction of Foxp3 expression in GPR83-transduced CD4+ T cells under inflammatory conditions. Our results suggest that GPR83 might be critically involved in the peripheral generation of Foxp3+ Treg cells in vivo.
- Subjects :
- Adoptive Transfer
Animals
Coculture Techniques
Dermatitis, Contact genetics
Dermatitis, Contact immunology
Dermatitis, Contact prevention & control
Forkhead Transcription Factors physiology
Humans
Lymphocyte Activation immunology
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Mice, Knockout
Mice, Transgenic
Receptors, G-Protein-Coupled administration & dosage
Receptors, G-Protein-Coupled biosynthesis
Resting Phase, Cell Cycle genetics
Retroviridae genetics
Retroviridae immunology
T-Lymphocyte Subsets immunology
T-Lymphocyte Subsets transplantation
T-Lymphocytes, Regulatory metabolism
T-Lymphocytes, Regulatory virology
Forkhead Transcription Factors biosynthesis
Lymphocyte Activation genetics
Receptors, G-Protein-Coupled genetics
Receptors, Interleukin-2 biosynthesis
Receptors, Interleukin-2 genetics
Resting Phase, Cell Cycle immunology
T-Lymphocytes, Regulatory immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1767
- Volume :
- 177
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of immunology (Baltimore, Md. : 1950)
- Publication Type :
- Academic Journal
- Accession number :
- 16785516
- Full Text :
- https://doi.org/10.4049/jimmunol.177.1.209