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Induction of oligodendrocyte progenitors in dorsal forebrain by intraventricular microinjection of FGF-2.
- Source :
-
Developmental biology [Dev Biol] 2006 Sep 01; Vol. 297 (1), pp. 262-73. Date of Electronic Publication: 2006 May 19. - Publication Year :
- 2006
-
Abstract
- During embryonic development, oligodendrocyte progenitors (OLPs) originate from the ventral forebrain under the regulation of Sonic hedgehog (Shh). Shh controls the expression of transcription factor Olig2, which is strongly implicated in OLP generation. Studies of mice deficient in Shh expression suggest, however, that an alternative pathway for OLP generation may exist. The generation of OLPs in dorsal forebrain has been suggested since treatment of dorsal-neural progenitor cells in culture with fibroblast growth factor (FGF-2) results in OLP induction. To ask if dorsal induction of OLPs in embryonic forebrain can occur in vivo and if FGF-2 could initiate an alternative pathway of regulation, we used in utero microinjection of FGF-2 into the lateral ventricles of mouse fetal forebrain. A single injection of FGF-2 at E13.5 resulted in the expression of the OLP markers Olig2 and PDGFRalpha mRNA in dorsal forebrain ventricular and intermediate zones. However, FGF-2 did not induce dorsal expression of Shh, Patched1 or Nkx2.1, and co-injection of FGF-2 and a Shh inhibitor did not attenuate the induction of Olig2 and PDGFRalpha, suggesting that Shh signaling was not involved in this FGF-2-mediated dorsal induction. These results demonstrate that the dorsal embryonic forebrain in vivo has the potential to generate OLPs in the presence of normal positional cues and that this can be driven by FGF-2 independent of Shh signaling.
- Subjects :
- Animals
Basic Helix-Loop-Helix Transcription Factors drug effects
Basic Helix-Loop-Helix Transcription Factors genetics
Cell Proliferation drug effects
Embryonic Induction drug effects
Embryonic Induction physiology
Epithelial Cells cytology
Epithelial Cells drug effects
Female
Fibroblast Growth Factor 2 pharmacology
Gene Expression Regulation, Developmental
Hedgehog Proteins
Homeodomain Proteins genetics
Mice
Mice, Inbred ICR
Microinjections
Nerve Tissue Proteins drug effects
Nerve Tissue Proteins genetics
Nuclear Proteins genetics
Oligodendrocyte Transcription Factor 2
Oligodendroglia drug effects
Patched Receptors
Patched-1 Receptor
Pregnancy
Prosencephalon drug effects
Receptor, Platelet-Derived Growth Factor alpha genetics
Receptors, Cell Surface genetics
Signal Transduction
Thyroid Nuclear Factor 1
Trans-Activators genetics
Transcription Factors genetics
Fibroblast Growth Factor 2 metabolism
Oligodendroglia physiology
Prosencephalon cytology
Prosencephalon embryology
Stem Cells drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 0012-1606
- Volume :
- 297
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Developmental biology
- Publication Type :
- Academic Journal
- Accession number :
- 16782086
- Full Text :
- https://doi.org/10.1016/j.ydbio.2006.05.017