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Temporal detection of equine herpesvirus infections of a cohort of mares and their foals.
- Source :
-
Veterinary microbiology [Vet Microbiol] 2006 Sep 10; Vol. 116 (4), pp. 249-57. Date of Electronic Publication: 2006 Jun 13. - Publication Year :
- 2006
-
Abstract
- The objectives of this study were to estimate the prevalence of equine herpesviruses (EHV) 1-5 in the nasal secretions (NS) of a cohort of 12 mares and their foals from birth to 6 months of age, estimate the prevalence of EHV-1-5 infection of peripheral blood mononuclear cells (PBMC) of selected foals, and investigate phylogenetic relationships amongst the various strains of EHV-2 and 5. Virus-specific PCR assays were used to detect EHV-1-5 in NS and PBMC. A homologous portion of the glycoprotein B (gB) gene of the various strains of EHV-2 and 5 was sequenced and compared. EHV-2, 4, and 5 were all detected in NS from the horses, but only EHV-4 was associated with respiratory disease (P=0.005). EHV-2 and 5 infections were both common, but foals shed EHV-2 in their NS earlier in life than EHV-5 (P=0.01). Latent EHV-2 and 5 infections were detected in the PBMC of 75 and 88%, respectively, of the foals at approximately 6 months of age. The strains of EHV-2 shed in the NS of individual horses were more genetically heterogeneous than the strains of EHV-5 (95.5-99.3% versus 98.8-99.3% nucleotide identity, respectively). One-month-old foals typically shed strains of EHV-2 that were identical to those infecting their dams whereas older foals often shed virus strains that were different from those of their dams. Although herpesvirus infections were ubiquitous in this cohort of horses, there were distinct clinical consequences and clear epidemiological differences between infections with the different viruses.
- Subjects :
- Aging immunology
Animals
Animals, Newborn
Base Sequence
Cohort Studies
DNA, Viral chemistry
DNA, Viral isolation & purification
Female
Herpesviridae Infections epidemiology
Herpesviridae Infections virology
Herpesvirus 1, Equid classification
Herpesvirus 1, Equid genetics
Herpesvirus 1, Equid isolation & purification
Herpesvirus 3, Equid classification
Herpesvirus 3, Equid genetics
Herpesvirus 3, Equid isolation & purification
Herpesvirus 4, Equid classification
Herpesvirus 4, Equid genetics
Herpesvirus 4, Equid isolation & purification
Horse Diseases virology
Horses
Leukocytes, Mononuclear virology
Molecular Sequence Data
Nasal Mucosa virology
Phylogeny
Polymerase Chain Reaction methods
Prevalence
Rhadinovirus classification
Rhadinovirus genetics
Species Specificity
Varicellovirus classification
Varicellovirus genetics
Herpesviridae Infections veterinary
Horse Diseases epidemiology
Polymerase Chain Reaction veterinary
Rhadinovirus isolation & purification
Varicellovirus isolation & purification
Subjects
Details
- Language :
- English
- ISSN :
- 0378-1135
- Volume :
- 116
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Veterinary microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 16774810
- Full Text :
- https://doi.org/10.1016/j.vetmic.2006.05.002