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Quantitative analysis of HIV-1 variants with the K103N resistance mutation after single-dose nevirapine in women with HIV-1 subtypes A, C, and D.
- Source :
-
Journal of acquired immune deficiency syndromes (1999) [J Acquir Immune Defic Syndr] 2006 Aug 15; Vol. 42 (5), pp. 610-3. - Publication Year :
- 2006
-
Abstract
- Introduction: We used a sensitive point mutation assay, LigAmp, to detect and quantify K103N-containing variants in African women who received single-dose nevirapine (NVP) to prevent mother-to-child HIV-1 transmission.<br />Methods: Plasma for testing was collected 6 to 8 weeks postpartum from 301 women (144 subtype A, 63 subtype C, and 94 subtype D).<br />Results: The portion of women with 0.5% or more K103N-containing variants was lowest for subtype A (60/144, 41.7%) and highest for subtype C (44/63, 69.8%; P < 0.0001). K103N was rarely detected in pre-NVP samples. In a multivariate model, K103N detection was associated with HIV-1 subtype (C > A), after adjusting for log10 delivery viral load, the number of days between NVP dosing and sample collection, age, and parity. Among women with K103N detected: (1) the median %K103N was lower for subtype A (2.2%) than C (11.7%, P = 0.0001) or D (5.5%, P = 0.04), and (2) in a multivariate linear model, higher log10 (%K103N) was associated with HIV subtype (C > A, P = 0.0001; D > A, P = 0.01; and C vs D, no difference), but not other factors.<br />Conclusions: After administration of single-dose NVP, K103N was detected more frequently and at higher levels in women with subtypes C and D than A. Further studies are needed to evaluate the clinical significance of NVP-resistant variants in this setting.
- Subjects :
- Amino Acid Substitution
Anti-HIV Agents therapeutic use
Drug Resistance, Viral genetics
Female
Genotype
HIV Infections drug therapy
HIV Infections transmission
HIV-1 classification
Humans
Infectious Disease Transmission, Vertical prevention & control
Malawi
Mutation, Missense
Nevirapine therapeutic use
Point Mutation
Pregnancy
Pregnancy Complications, Infectious drug therapy
Statistics as Topic
Uganda
Anti-HIV Agents pharmacology
HIV Infections virology
HIV-1 drug effects
HIV-1 genetics
Nevirapine pharmacology
Pregnancy Complications, Infectious virology
Subjects
Details
- Language :
- English
- ISSN :
- 1525-4135
- Volume :
- 42
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Journal of acquired immune deficiency syndromes (1999)
- Publication Type :
- Academic Journal
- Accession number :
- 16773030
- Full Text :
- https://doi.org/10.1097/01.qai.0000221686.67810.20