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Correlation between serological immune response analyzed by a new ELISA for HPV-16/18 E7 oncoprotein and clinical characteristics of cervical cancer patients.
- Source :
-
Archives of virology [Arch Virol] 2006 Oct; Vol. 151 (10), pp. 1899-916. Date of Electronic Publication: 2006 May 29. - Publication Year :
- 2006
-
Abstract
- Human papillomaviruses (HPVs), particularly HPV-16/18, are linked to cervical cancer development. Full-length, recombinant HPV-16/18 E7 oncoproteins were used in a new streptavidin-biotin capture ELISA method to investigate anti-HPV E7 antibody prevalence in serum. Sera from 99 healthy women, 70 cervical cancer patients, and 30 patients with cervical pre-invasive neoplasia were analyzed. Anti-HPV-16/18 E7 positivity was found in 53% of cervical cancer patients, in 40% with cervical pre-invasive neoplasia, and in 8% of healthy women. Serum samples from 12 cervical cancer patients were obtained at different time intervals during the treatment. Eleven out of 12 showed a correspondence between HPV-E7 antibody levels (decreasing versus increasing) and the type of response (clinically complete or partial response versus progression or stable disease) at each serological evaluation. Five patients with recurrent HPV-16/18-positive cervical carcinoma were analyzed before and after vaccination with HPV-16/18 E7-pulsed autologous dendritic cells; anti-HPV-16/18 E7 positivity was found in 3 out of 5 women. In conclusion, this assay could potentially be used as an adjunctive tool to monitor the type of response to treatment and possibly to detect antibody induction in cervical cancer patients after vaccination, as a potential marker to evaluate its efficacy.
- Subjects :
- Antibody Specificity
Biomarkers blood
Biotin
Cancer Vaccines administration & dosage
DNA-Binding Proteins biosynthesis
Disease Progression
Female
Humans
Immunoglobulin G immunology
Oncogene Proteins, Viral biosynthesis
Papillomavirus E7 Proteins
Papillomavirus Vaccines administration & dosage
Recombinant Proteins biosynthesis
Recombinant Proteins immunology
Sensitivity and Specificity
Streptavidin
Vaccination
Antibodies, Viral blood
Carcinoma blood
Carcinoma diagnosis
DNA-Binding Proteins immunology
Enzyme-Linked Immunosorbent Assay methods
Immunoglobulin G blood
Oncogene Proteins, Viral immunology
Precancerous Conditions blood
Precancerous Conditions diagnosis
Uterine Cervical Neoplasms blood
Uterine Cervical Neoplasms diagnosis
Subjects
Details
- Language :
- English
- ISSN :
- 0304-8608
- Volume :
- 151
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Archives of virology
- Publication Type :
- Academic Journal
- Accession number :
- 16732494
- Full Text :
- https://doi.org/10.1007/s00705-006-0787-y