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The effect of down-regulation of mitochondrial RNA-binding proteins MRP1 and MRP2 on respiratory complexes in procyclic Trypanosoma brucei.
- Source :
-
Molecular and biochemical parasitology [Mol Biochem Parasitol] 2006 Sep; Vol. 149 (1), pp. 65-73. Date of Electronic Publication: 2006 May 15. - Publication Year :
- 2006
-
Abstract
- MRP1 and MRP2 are multifunctional mitochondrial RNA-binding proteins with a regulatory role in RNA editing and putative role(s) in RNA processing in Trypanosoma brucei. Silencing of MRP1 and/or MRP2 by RNA interference affected the assembly and functionality of respiratory complexes. The absence of several subunits of complexes I, III and IV resulted in their disintegration and subsequent decrease of specific activities and also caused a significant decrease of membrane potential. The overall respiration in the interfered cells decreased by only about 20%, since the trypanosome alternative oxidase effectively replaced the missing cytochromes and became the principal terminal oxidase.
- Subjects :
- Animals
Down-Regulation
Electron Transport
Electron Transport Complex IV genetics
Electron Transport Complex IV metabolism
Mitochondrial Proteins metabolism
Proton-Translocating ATPases genetics
RNA Editing
RNA Interference
RNA-Binding Proteins metabolism
Trypanosoma brucei brucei genetics
Electron Transport Chain Complex Proteins metabolism
Mitochondria metabolism
Mitochondrial Proteins genetics
RNA-Binding Proteins genetics
Trypanosoma brucei brucei metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0166-6851
- Volume :
- 149
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Molecular and biochemical parasitology
- Publication Type :
- Academic Journal
- Accession number :
- 16730807
- Full Text :
- https://doi.org/10.1016/j.molbiopara.2006.04.007