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Investigation of HER-2 codon 655 single nucleotide polymorphism frequency and c-ErbB-2 protein expression alterations in gastric cancer patients.
- Source :
-
World journal of gastroenterology [World J Gastroenterol] 2006 May 28; Vol. 12 (20), pp. 3283-7. - Publication Year :
- 2006
-
Abstract
- Aim: To investigate both whether the risk of gastric cancer is associated with the Ile/Val single nucleotide polymorphism (SNP) of human epidermal growth factor receptor-2 (HER-2) transmembrane domain-coding region at codon 655 and the suggested existence of HER-2 expression in gastric cancer cases in a Turkish patient group.<br />Methods: Polymerase chain reaction (PCR) followed by restriction fragment length polymorphism (RFLP) strategy was used to analyze the presence of HER-2 SNP at codon 655. c-erbB-2 expression pattern was analyzed by immunohistochemistry. The results were compared between gastric carcinoma group and chronic gastritis group, as well as between clinicopathological parameters and carcinoma.<br />Results: Results showed that Ile/Val genotype accounted for 20% within the Turkish gastric carcinoma group, and none in chronic gastritis group, and this genotyping was associated with stage IV gastric cancers (P=0.04). Positive membranous HER-2 immunoreactivity, on the other hand, accounted for 24% within the Turkish gastric carcinoma group and none from chronic gastritis cases; further, it was correlated with intestinal type carcinomas (P=0.007), and stage III-IV carcinomas (P=0.004).<br />Conclusion: These observations imply that the tested HER-2 SNP may participate in the development and progression of gastric cancer. Thus, after confirming these results with large sample groups, HER-2 codon 655 SNP and/or c-erbB-2 overexpression may also be used as a poor prognostic indicator for gastric carcinomas.
- Subjects :
- Adult
Aged
Aged, 80 and over
Carcinoma, Adenosquamous chemistry
Case-Control Studies
Female
Gastritis genetics
Genetic Predisposition to Disease
Humans
Male
Middle Aged
Predictive Value of Tests
Prognosis
Receptor, ErbB-2 analysis
Risk Factors
Stomach Neoplasms chemistry
Turkey
Carcinoma, Adenosquamous genetics
Codon genetics
Gene Expression Regulation, Neoplastic genetics
Genes, erbB-2 genetics
Polymorphism, Single Nucleotide
Receptor, ErbB-2 genetics
Stomach Neoplasms genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1007-9327
- Volume :
- 12
- Issue :
- 20
- Database :
- MEDLINE
- Journal :
- World journal of gastroenterology
- Publication Type :
- Academic Journal
- Accession number :
- 16718853
- Full Text :
- https://doi.org/10.3748/wjg.v12.i20.3283