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Genes associated with adult axon regeneration promoted by olfactory ensheathing cells: a new role for matrix metalloproteinase 2.
- Source :
-
The Journal of neuroscience : the official journal of the Society for Neuroscience [J Neurosci] 2006 May 17; Vol. 26 (20), pp. 5347-59. - Publication Year :
- 2006
-
Abstract
- The molecular mechanisms used by olfactory ensheathing cells (OECs) to promote repair in the damaged adult mammalian CNS remain unknown. Thus, we used microarrays to analyze three OEC populations with different capacities to promote axonal regeneration in cultured rat retinal neurons. Gene expression in "long-term cultured OECs" that do not stimulate adult axonal outgrowth was compared with that of "primary olfactory ensheathing cells" and the immortalized OEC cell line TEG3. In this way, we identified a number of candidate genes that might play a role in promoting adult axonal regeneration. Among these genes, it was striking that both the matrix metalloproteinase 2 (MMP2) and an inhibitor of this protease were represented. The disruption of MMP2 activity in TEG3 cells impaired their capacity to trigger axon regeneration in cultured adult retinal neurons. Furthermore, the MMP2 protein was detected in grafts of OECs that elicited robust axonal regeneration in the injured spinal cord of adult rats in vivo. These data suggest that MMP2 does indeed participate in adult axonal regeneration induced by OECs.
- Subjects :
- Animals
Animals, Newborn
Brain Tissue Transplantation methods
Cell Culture Techniques methods
Cell Line, Transformed
Cells, Cultured
Coculture Techniques
Enzyme Inhibitors pharmacology
Growth Cones ultrastructure
Male
Matrix Metalloproteinase 2 genetics
Matrix Metalloproteinase Inhibitors
Neuroglia cytology
Neuroglia transplantation
Olfactory Bulb cytology
Olfactory Bulb metabolism
Olfactory Bulb transplantation
Oligonucleotide Array Sequence Analysis
Rats
Rats, Wistar
Retinal Ganglion Cells cytology
Retinal Ganglion Cells metabolism
Spinal Cord cytology
Spinal Cord metabolism
Spinal Cord Injuries metabolism
Spinal Cord Injuries physiopathology
Spinal Cord Injuries therapy
Gene Expression Regulation genetics
Growth Cones metabolism
Matrix Metalloproteinase 2 metabolism
Nerve Regeneration genetics
Neuroglia metabolism
Neuronal Plasticity genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1529-2401
- Volume :
- 26
- Issue :
- 20
- Database :
- MEDLINE
- Journal :
- The Journal of neuroscience : the official journal of the Society for Neuroscience
- Publication Type :
- Academic Journal
- Accession number :
- 16707787
- Full Text :
- https://doi.org/10.1523/JNEUROSCI.1111-06.2006