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Identification and characterization of polymorphisms within the 5' flanking region, first exon and part of first intron of bovine GH gene.
- Source :
-
Journal of animal breeding and genetics = Zeitschrift fur Tierzuchtung und Zuchtungsbiologie [J Anim Breed Genet] 2006 Jun; Vol. 123 (3), pp. 208-12. - Publication Year :
- 2006
-
Abstract
- The aim of the present study was to identify and characterize polymorphisms within the 5' flanking region, first exon and part of first intron of the bovine growth hormone gene among different beef cattle breeds: Nelore (n = 25), Simmental (n = 39), Simbrasil (n = 24), Simmental x Nelore (n = 30), Canchim x Nelore (n = 30) and Angus x Nelore (n = 30). Two DNA fragments (GH1, 464 bp and GH2, 453 bp) were amplified by polymerase chain reaction and then used for polymorphism identification by SSCP. Within the GH1 fragment, five polymorphisms were identified, corresponding to three different alleles: GH1.1, GH1.2 and GH1.3 (GenBank: AY662648, AY662649 and AY662650, respectively). These allele sequences were aligned and compared with bovine GH gene nucleotide sequence (GenBank: M57764 and AF118837), resulting in the identification of five insertion/deletions (INDELs) and five single nucleotide polymorphisms (SNPs). In the GH2 fragment two alleles were identified, GH2.1 and GH2.2 (GenBank: AY662651 and AY662652, respectively). The allele sequences were compared with GenBank sequences (M57764, AF007750 and AH009106) and three INDELs and four SNPs were identified. In conclusion, we were able to identify six new polymorphisms of the bovine GH gene (one INDEL and five SNPs), which can be used as molecular markers in genetic studies.
- Subjects :
- Alleles
Animals
Cattle classification
DNA Primers chemistry
Exons genetics
Gene Frequency
Gene Order
Introns genetics
Molecular Sequence Data
Polymorphism, Single-Stranded Conformational
Sequence Alignment veterinary
5' Flanking Region genetics
Cattle genetics
Genetic Markers genetics
Growth Hormone genetics
Polymorphism, Genetic genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0931-2668
- Volume :
- 123
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of animal breeding and genetics = Zeitschrift fur Tierzuchtung und Zuchtungsbiologie
- Publication Type :
- Academic Journal
- Accession number :
- 16706927
- Full Text :
- https://doi.org/10.1111/j.1439-0388.2006.00574.x