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Pre-B-cell colony-enhancing factor (PBEF/Visfatin) gene expression is modulated by NF-kappaB and AP-1 in human amniotic epithelial cells.
- Source :
-
Placenta [Placenta] 2007 Apr; Vol. 28 (4), pp. 305-14. Date of Electronic Publication: 2006 May 15. - Publication Year :
- 2007
-
Abstract
- A localized intrauterine inflammatory response is often associated with the initiation of normal human parturition, whereas infection causes a similar but more florid response initiating preterm labor. Pre-B-cell colony-enhancing factor (PBEF) is expressed in the human fetal membranes and is up-regulated by labor, severe infection and inflammatory stimuli. The aim of this study was to determine the involvement of the transcription factors NF-kappaB and AP-1 in the response of PBEF to an inflammatory stimulus and compare it with IL-8. The results showed that this treatment of amniotic epithelial-like cells (WISH) and primary amniotic epithelial cells increased expression of PBEF and IL-8, but IL-8 responded 100-fold more than PBEF. IL-1beta treatment together with a panel of NF-kappaB and AP-1 inhibitors demonstrated the involvement of these transcription factors in the up-regulation of PBEF. These data show that an inflammatory stimulus in the fetal membranes inducing NF-kappaB and AP-1 would up-regulate PBEF as well as IL-8.
- Subjects :
- Amnion cytology
Amnion metabolism
Cell Count
Cell Nucleus drug effects
Cells, Cultured
Cytokines metabolism
Dose-Response Relationship, Drug
Epithelial Cells cytology
Epithelial Cells metabolism
Humans
Interleukin-1beta pharmacology
Interleukin-8 metabolism
Nicotinamide Phosphoribosyltransferase
Up-Regulation
Amnion drug effects
Cytokines genetics
Epithelial Cells drug effects
Gene Expression Regulation, Developmental drug effects
NF-kappa B pharmacology
Transcription Factor AP-1 pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0143-4004
- Volume :
- 28
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Placenta
- Publication Type :
- Academic Journal
- Accession number :
- 16701870
- Full Text :
- https://doi.org/10.1016/j.placenta.2006.03.011