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Pre-B-cell colony-enhancing factor (PBEF/Visfatin) gene expression is modulated by NF-kappaB and AP-1 in human amniotic epithelial cells.

Authors :
Kendal CE
Bryant-Greenwood GD
Source :
Placenta [Placenta] 2007 Apr; Vol. 28 (4), pp. 305-14. Date of Electronic Publication: 2006 May 15.
Publication Year :
2007

Abstract

A localized intrauterine inflammatory response is often associated with the initiation of normal human parturition, whereas infection causes a similar but more florid response initiating preterm labor. Pre-B-cell colony-enhancing factor (PBEF) is expressed in the human fetal membranes and is up-regulated by labor, severe infection and inflammatory stimuli. The aim of this study was to determine the involvement of the transcription factors NF-kappaB and AP-1 in the response of PBEF to an inflammatory stimulus and compare it with IL-8. The results showed that this treatment of amniotic epithelial-like cells (WISH) and primary amniotic epithelial cells increased expression of PBEF and IL-8, but IL-8 responded 100-fold more than PBEF. IL-1beta treatment together with a panel of NF-kappaB and AP-1 inhibitors demonstrated the involvement of these transcription factors in the up-regulation of PBEF. These data show that an inflammatory stimulus in the fetal membranes inducing NF-kappaB and AP-1 would up-regulate PBEF as well as IL-8.

Details

Language :
English
ISSN :
0143-4004
Volume :
28
Issue :
4
Database :
MEDLINE
Journal :
Placenta
Publication Type :
Academic Journal
Accession number :
16701870
Full Text :
https://doi.org/10.1016/j.placenta.2006.03.011