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Increase in synaptic hippocampal zinc concentration following chronic but not acute zinc treatment in rats.
- Source :
-
Brain research [Brain Res] 2006 May 23; Vol. 1090 (1), pp. 69-75. Date of Electronic Publication: 2006 May 03. - Publication Year :
- 2006
-
Abstract
- Electroconvulsive seizures (ECS), one of the most effective treatments of depression, induce mossy fiber sprouting (when assayed by means of synaptic zinc method), and this indicates an increase in the synaptic zinc level in the hippocampus following such therapy. The aim of the present study was to investigate the influence of acute and chronic zinc hydroaspartate administration on the synaptic and total zinc level in the rat hippocampus. We used two methods of zinc determination: (1) zinc-selenium method, which images the pool of synaptic zinc, and (2) flame atomic absorption spectrometry, which assays the total concentration of zinc. Our results indicate that chronic (14 x 65 mg/kg), but not acute, zinc hydroaspartate administration intraperitoneally (i.p.) increases the pool of synaptic zinc in the majority of rat hippocampal layers (by 72-190%), except for the stratum moleculare and stratum radiatum CA, and perforant path DG. On the other hand, no changes were found in total hippocampal zinc level, measured by flame atomic absorption spectrometry. These data suggest that chronic zinc treatment increases the pool of synaptic zinc in the hippocampus, and this effect is similar to that observed following chronic ECS treatment. The measurement of zinc concentration in the whole hippocampus by the flame atomic absorption spectrometry method is not sensitive enough to detect such subtle alteration.
- Subjects :
- Animals
Dose-Response Relationship, Drug
Drug Administration Schedule
Electroshock
Growth Cones metabolism
Growth Cones ultrastructure
Hippocampus anatomy & histology
Injections, Intraperitoneal
Male
Neuronal Plasticity physiology
Predictive Value of Tests
Rats
Rats, Wistar
Spectrophotometry, Atomic
Synaptic Transmission drug effects
Synaptic Transmission physiology
Up-Regulation drug effects
Up-Regulation physiology
Zinc analysis
Zinc Compounds analysis
Zinc Compounds metabolism
Zinc Compounds pharmacology
Hippocampus drug effects
Hippocampus metabolism
Presynaptic Terminals drug effects
Presynaptic Terminals metabolism
Zinc metabolism
Zinc pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0006-8993
- Volume :
- 1090
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Brain research
- Publication Type :
- Academic Journal
- Accession number :
- 16674928
- Full Text :
- https://doi.org/10.1016/j.brainres.2006.03.035