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Indirect fluorescent antibody testing of cerebrospinal fluid for diagnosis of equine protozoal myeloencephalitis.
- Source :
-
American journal of veterinary research [Am J Vet Res] 2006 May; Vol. 67 (5), pp. 869-76. - Publication Year :
- 2006
-
Abstract
- Objective: To assess the use of CSF testing with an indirect fluorescent antibody test (IFAT) for diagnosis of equine protozoal myeloencephalitis (EPM) caused by Sarcocystis neurona.<br />Sample Population: Test results of 428 serum and 355 CSF samples from 182 naturally exposed, experimentally infected, or vaccinated horses.<br />Procedure: EPM was diagnosed on the basis of histologic examination of the CNS. Probability distributions were fitted to serum IFAT results in the EPM+ and EPM-horses, and correlation between serum and CSF results was modeled. Pairs of serum-CSF titers were generated by simulation, and titer-specific likelihood ratios and post-test probabilities of EPM at various pretest probability values were estimated. Post-test probabilities were compared for use of a serum-CSF test combination, a serum test only, and a CSF test only.<br />Results: Post-test probabilities of EPM increased as IFAT serum and CSF titers increased. Post-test probability differences for use of a serum-CSF combination and a serum test only were < or = 19% in 95% of simulations. The largest increases occurred when serum titers were from 40 to 160 and pre-test probabilities were from 5% to 60%. In all simulations, the difference between pre- and post-test probabilities was greater for a CSF test only, compared with a serum test only.<br />Conclusions and Clinical Relevance: CSF testing after a serum test has limited usefulness in the diagnosis of EPM. A CSF test alone might be used when CSF is required for other procedures. Ruling out other causes of neurologic disease reduces the necessity of additional EPM testing.
- Subjects :
- Animals
Central Nervous System Protozoal Infections cerebrospinal fluid
Central Nervous System Protozoal Infections diagnosis
Horses
Sarcocystosis cerebrospinal fluid
Sarcocystosis diagnosis
Antibodies, Protozoan cerebrospinal fluid
Central Nervous System Protozoal Infections veterinary
Fluorescent Antibody Technique, Indirect veterinary
Horse Diseases cerebrospinal fluid
Horse Diseases diagnosis
Sarcocystosis veterinary
Subjects
Details
- Language :
- English
- ISSN :
- 0002-9645
- Volume :
- 67
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- American journal of veterinary research
- Publication Type :
- Academic Journal
- Accession number :
- 16649923
- Full Text :
- https://doi.org/10.2460/ajvr.67.5.869