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Delayed Schwann cell and oligodendrocyte remyelination after ethidium bromide injection in the brainstem of Wistar rats submitted to streptozotocin diabetogenic treatment.
- Source :
-
Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas [Braz J Med Biol Res] 2006 May; Vol. 39 (5), pp. 637-46. Date of Electronic Publication: 2006 Apr 20. - Publication Year :
- 2006
-
Abstract
- Schwann cell disturbance followed by segmental demyelination in the peripheral nervous system occurs in diabetic patients. Since Schwann cell and oligodendrocyte remyelination in the central nervous system is a well-known event in the ethidium bromide (EB) demyelinating model, the aim of this investigation was to determine the behavior of both cell types after local EB injection into the brainstem of streptozotocin diabetic rats. Adult male Wistar rats received a single intravenous injection of streptozotocin (50 mg/kg) and were submitted 10 days later to a single injection of 10 microL 0.1% (w/v) EB or 0.9% saline solution into the cisterna pontis. Ten microliters of 0.1% EB was also injected into non-diabetic rats. The animals were anesthetized and perfused through the heart 7 to 31 days after EB or saline injection and brainstem sections were collected and processed for light and transmission electron microscopy. The final balance of myelin repair in diabetic and non-diabetic rats at 31 days was compared using a semi-quantitative method. Diabetic rats presented delayed macrophage activity and lesser remyelination compared to non-diabetic rats. Although oligodendrocytes were the major remyelinating cells in the brainstem, Schwann cells invaded EB-induced lesions, first appearing at 11 days in non-diabetic rats and by 15 days in diabetic rats. Results indicate that short-term streptozotocin-induced diabetes hindered both oligodendrocyte and Schwann cell remyelination (mean remyelination scores of 2.57 +/- 0.77 for oligodendrocytes and 0.67 +/- 0.5 for Schwann cells) compared to non-diabetic rats (3.27 +/- 0.85 and 1.38 +/- 0.81, respectively).
- Subjects :
- Animals
Brain Stem ultrastructure
Demyelinating Diseases chemically induced
Male
Microscopy, Electron, Transmission
Myelin Sheath physiology
Nerve Regeneration physiology
Oligodendroglia physiology
Oligodendroglia ultrastructure
Rats
Rats, Wistar
Schwann Cells physiology
Schwann Cells ultrastructure
Time Factors
Brain Stem drug effects
Demyelinating Diseases physiopathology
Diabetes Mellitus, Experimental physiopathology
Ethidium toxicity
Myelin Sheath drug effects
Oligodendroglia drug effects
Schwann Cells drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 0100-879X
- Volume :
- 39
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas
- Publication Type :
- Academic Journal
- Accession number :
- 16648902
- Full Text :
- https://doi.org/10.1590/s0100-879x2006000500011