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Alteration of nitrergic neuromuscular transmission as a result of acute experimental colitis in rat.
- Source :
-
Journal of veterinary science [J Vet Sci] 2006 Jun; Vol. 7 (2), pp. 143-50. - Publication Year :
- 2006
-
Abstract
- Nitric oxide (NO) is a non-adrenergic, non-cholinergic neurotransmitter found in the enteric nervous system that plays a role in a variety of enteropathies, including inflammatory bowel disease. Alteration of nitrergic neurons has been reported to be dependent on the manner by which inflammation is caused. However, this observed alteration has not been reported with acetic acid-induced colitis. Therefore, the purpose of the current study was to investigate changes in nitrergic neuromuscular transmission in experimental colitis in a rat model. Distal colitis was induced by intracolonic administration of 4 % acetic acid in the rat. Animals were sacrificed at 4 h and 48 h postacetic acid treatment. Myeloperoxidase activity was significantly increased in the acetic acid-treated groups. However, the response to 60 mM KCl was not significantly different in the three groups studied. The amplitude of phasic contractions was increased by Nomega-nitro-L-arginine methyl ester (L-NAME) in the normal control group, but not in the acetic acid-treated groups. Spontaneous contractions disappeared during electrical field stimulation (EFS) in normal group. However, for the colitis groups, these contractions initially disappeared, and then reappeared during EFS. Moreover, the observed disappearance was diminished by L-NAME; this suggests that these responses were NO-mediated. In addition, the number of NADPH-diaphorase positive nerve cell bodies, in the myenteric plexus, was not altered in the distal colon; whereas the area of NADPH-diaphorase positive fibers, in the circular muscle layer, was decreased in the acetic acidtreated groups. These results suggest that NO-mediated inhibitory neural input, to the circular muscle, was decreased in the acetic acid-treated groups.
- Subjects :
- Acetic Acid toxicity
Animals
Colitis chemically induced
Colon drug effects
Colon enzymology
Colon pathology
Indicators and Reagents toxicity
Male
Muscle Contraction drug effects
Muscle, Smooth drug effects
Muscle, Smooth metabolism
Myenteric Plexus pathology
NADPH Dehydrogenase metabolism
NG-Nitroarginine Methyl Ester pharmacology
Neuromuscular Junction drug effects
Nitrergic Neurons drug effects
Peroxidase metabolism
Potassium Chloride pharmacology
Rats
Rats, Sprague-Dawley
Colitis pathology
Colitis physiopathology
Colon innervation
Neuromuscular Junction metabolism
Nitrergic Neurons metabolism
Nitric Oxide metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1229-845X
- Volume :
- 7
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Journal of veterinary science
- Publication Type :
- Academic Journal
- Accession number :
- 16645339
- Full Text :
- https://doi.org/10.4142/jvs.2006.7.2.143