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Transcriptomics tool for the human Schistosoma blood flukes using microarray gene expression profiling.
- Source :
-
Experimental parasitology [Exp Parasitol] 2006 Nov; Vol. 114 (3), pp. 160-72. Date of Electronic Publication: 2006 Apr 24. - Publication Year :
- 2006
-
Abstract
- We report the design, fabrication, and validation of a microarray covering the majority of the Schistosoma japonicum and Schistosoma mansoni transcriptomes. The oligonucleotide microarray contains 12,166 S. mansoni and 7055 S. japonicum target sequences. A confidence threshold of <or=0.001 (P value) was used in all analyses. The cross hybridization potential of the microarray was representative of 10,362 probes detected in both channels, while 12,052 probes hybridised to S. japonicum and 11,254 probes hybridised to S. mansoni. Differential hybridisation consisted of 3422 probes to S. mansoni mRNA and 3103 probes to S. japonicum mRNA. Important biological functions, such as transport, metabolism, immune evasion and host-parasite immunological interplay, cell communication, and sexual maturation are represented on this microarray. This is the first microarray commercially manufactured for studying schistosomes, and the large size and verified quality of the resource demonstrate its power for characterising the schistosome transcriptome.
- Subjects :
- Animals
Biomphalaria
Female
Fertility genetics
Gene Expression Regulation genetics
Host-Parasite Interactions immunology
In Situ Hybridization, Fluorescence
Male
Mice
Oligonucleotide Array Sequence Analysis standards
RNA, Helminth analysis
RNA, Helminth genetics
RNA, Messenger analysis
RNA, Messenger genetics
Reverse Transcriptase Polymerase Chain Reaction
Schistosoma japonicum immunology
Schistosoma japonicum physiology
Schistosoma mansoni immunology
Schistosoma mansoni physiology
Signal Transduction genetics
Gene Expression Profiling methods
Oligonucleotide Array Sequence Analysis methods
Schistosoma japonicum genetics
Schistosoma mansoni genetics
Transcription, Genetic
Subjects
Details
- Language :
- English
- ISSN :
- 0014-4894
- Volume :
- 114
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Experimental parasitology
- Publication Type :
- Academic Journal
- Accession number :
- 16631746
- Full Text :
- https://doi.org/10.1016/j.exppara.2006.03.003