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Increased RhoA translocation in aorta of diabetic rats.
- Source :
-
Acta pharmacologica Sinica [Acta Pharmacol Sin] 2006 May; Vol. 27 (5), pp. 543-8. - Publication Year :
- 2006
-
Abstract
- Aim: To analyze RhoA expression and activation in the aorta of diabetic rats.<br />Methods: Male SD rats (n=70) were divided into 2 groups: the diabetic group and the control group. Diabetes was induced by intravenous injection of streptozotocin (55 mg/kg). The Rats were studied 3 weeks after the induction of diabetes. Western blotting was used to measure the expression and activation of Rho.<br />Results: Heart rate was measured 24 h/d; it decreased by 58+/-13 beats/min in the diabetic rats. Isometric tension showed that the contraction of diabetic aorta was significantly reduced compared with that of control aorta when stimulated by KCl and serotonin. The relaxation of the diabetic aorta was reduced when stimulated by acetylcholine. An enhanced RhoA translocation in the aortic tissues of diabetic rats was determined by a 90% increase in membrane-bound RhoA, indicating that the activation of RhoA is markedly increased in the diabetic aorta.<br />Conclusion: Our data suggest that upregulated RhoA could be involved in the vascular dysfunction of diabetic rats.
- Subjects :
- Acetylcholine pharmacology
Animals
Aorta physiopathology
Diabetes Mellitus, Experimental chemically induced
Diabetes Mellitus, Experimental physiopathology
Enzyme Activation
Heart Rate
Male
Muscle Contraction drug effects
Muscle Relaxation drug effects
Muscle, Smooth, Vascular physiopathology
Potassium Chloride pharmacology
Protein Transport
RNA, Messenger genetics
RNA, Messenger metabolism
Rats
Rats, Sprague-Dawley
Streptozocin
Up-Regulation
rhoA GTP-Binding Protein genetics
Aorta metabolism
Diabetes Mellitus, Experimental metabolism
rhoA GTP-Binding Protein metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1671-4083
- Volume :
- 27
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Acta pharmacologica Sinica
- Publication Type :
- Academic Journal
- Accession number :
- 16626508
- Full Text :
- https://doi.org/10.1111/j.1745-7254.2006.00325.x