Back to Search
Start Over
Vaccination with human tyrosinase DNA induces antibody responses in dogs with advanced melanoma.
- Source :
-
Cancer immunity [Cancer Immun] 2006 Apr 21; Vol. 6, pp. 8. Date of Electronic Publication: 2006 Apr 21. - Publication Year :
- 2006
-
Abstract
- Antitumor immune responses can be elicited in preclinical mouse melanoma models using plasmid DNA vaccines encoding xenogeneic melanosomal differentiation antigens. We previously reported on a phase I clinical trial of human tyrosinase (huTyr) DNA vaccination of 9 dogs with advanced malignant melanoma (World Health Organization stages II-IV), in which we demonstrated the safety of the treatment and the prolongation of the expected survival time (ST) of subjects as compared to historical, stage-matched controls. As a secondary goal of the same study, we report here on the induction of tyrosinase-specific antibody responses in three of the nine dogs vaccinated with huTyr DNA. The antibodies in two of the three responders cross-react with syngeneic canine tyrosinase, demonstrating the ability of this vaccine to overcome host immune tolerance and/or ignorance to or of "self" antigens. Most interestingly, the onset of antibody induction in these three dogs coincides with observed clinical responses and may suggest a means to account for their long-term tumor control and survival.
- Subjects :
- Amino Acid Sequence
Animals
Antigens, Heterophile genetics
Antigens, Heterophile immunology
Autoantibodies blood
Cancer Vaccines genetics
Cell Line, Tumor
Conserved Sequence
Dog Diseases pathology
Dogs
Enzyme-Linked Immunosorbent Assay
Flow Cytometry
Humans
Melanoma immunology
Melanoma pathology
Molecular Sequence Data
Monophenol Monooxygenase chemistry
Monophenol Monooxygenase genetics
Sequence Homology, Amino Acid
Survival Analysis
Vaccines, DNA immunology
Antibodies blood
Cancer Vaccines immunology
Dog Diseases immunology
Melanoma veterinary
Monophenol Monooxygenase immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1424-9634
- Volume :
- 6
- Database :
- MEDLINE
- Journal :
- Cancer immunity
- Publication Type :
- Academic Journal
- Accession number :
- 16626110