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Protein kinase C activating phorbolesters enhance the cyclic AMP response to parathyroid hormone, forskolin and choleratoxin in mouse calvarial bones and rat osteosarcoma cells.
- Source :
-
Bioscience reports [Biosci Rep] 1991 Aug; Vol. 11 (4), pp. 203-11. - Publication Year :
- 1991
-
Abstract
- The protein kinase C-(PKC) activating phorbol esters 12-O-tetradecanoylphorbol-13-acetate (TPA; 100 nmol/l) and phorbol 12,13-dibutyrate (PDBU; 100 nmol/l) enhanced basal cyclin AMP accumulation in cultured neonatal mouse calvaria. The cyclic AMP response to parathyroid hormone (PTH; 10 nmol/l) and the adenylate cyclase activators forskolin (1-3 mumol/l) and choleratoxin (0.1 mumg/ml) was potentiated in a more than additive manner by TPA and PDBU. In contrast, phorbol 13-monoacetate (phorb-13; 100 nmol/l), a related compound but inactive on PKC, had no effect on basal or stimulated cyclic AMP accumulation. In the presence of indomethacin (1 mumol/l), TPA and PDBU had no effect on cyclic AMP accumulation in calvarial bones per se, but were still able to cause a significant enhancement of the response to PTH, forskolin and choleratoxin. PTH-, forskolin- and choleratoxin-stimulated cyclic AMP accumulation in rat osteosarcoma cells UMR 106-01 was synergistically potentiated by TPA and PDBU, but not by phorb.-13. These data indicate that PKC enhances cyclic AMP formation and that the level of interaction may be at, or distal to, adenylate cyclase.
- Subjects :
- Adenylyl Cyclases metabolism
Animals
Bone and Bones metabolism
Cattle
Cholera Toxin pharmacology
Colforsin pharmacology
Culture Techniques
Enzyme Activation drug effects
Mice
Osteosarcoma
Parathyroid Hormone physiology
Phorbol 12,13-Dibutyrate pharmacology
Rats
Tetradecanoylphorbol Acetate pharmacology
Tumor Cells, Cultured
Cyclic AMP metabolism
Phorbol Esters pharmacology
Protein Kinase C metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0144-8463
- Volume :
- 11
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Bioscience reports
- Publication Type :
- Academic Journal
- Accession number :
- 1662087
- Full Text :
- https://doi.org/10.1007/BF01136854