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More than colocalizing with polycystin-1, polycystin-L is in the centrosome.
- Source :
-
American journal of physiology. Renal physiology [Am J Physiol Renal Physiol] 2006 Aug; Vol. 291 (2), pp. F395-406. Date of Electronic Publication: 2006 Apr 11. - Publication Year :
- 2006
-
Abstract
- Polycystin-1 and polycystin-2 are involved in autosomal dominant polycystic kidney disease by unknown mechanisms. These two proteins are located in primary cilia where they mediate mechanosensation, suggesting a link between cilia function and renal disease. In this study, we sought to characterize the subcellular localization of polycystin-L, a closely related member of polycystin-2, in epithelial renal cell lines. We have shown that endogenous polycystin-l subcellular distribution is different in proliferative and nonproliferative cultures. Polycystin-L is found mostly in the endoplasmic reticulum in subconfluent cell cultures, while in confluent cells it is redistributed to sites of cell-cell contact and to the primary cilium as is polycystin-1. Subcellular fractionation confirmed a common distribution of polycystin-L and polycystin-1 in the fractions corresponding to those containing the plasma membrane of postconfluent cells. Reciprocal coimmunoprecipitation experiments showed that polycystin-L was associated with polycystin-1 in a common complex in both subconfluent and confluent cell cultures. Interestingly, we also identified a novel site for a polycystin member (polycystin-L) in unciliated cells, the centrosome, which allowed us to reveal an involvement of polycystin-l in cell proliferation.
- Subjects :
- Animals
Calcium Channels
Cell Line
Cell Membrane chemistry
Cell Membrane physiology
Cell Proliferation
Centrosome physiology
Cilia chemistry
Cilia physiology
Dogs
Endoplasmic Reticulum chemistry
Endoplasmic Reticulum physiology
Epithelial Cells chemistry
Epithelial Cells cytology
Epithelial Cells physiology
Immunohistochemistry
Kidney chemistry
Kidney cytology
Kidney physiology
Kidney Tubules, Collecting chemistry
Kidney Tubules, Collecting cytology
Kidney Tubules, Collecting physiology
Membrane Glycoproteins analysis
Mice
Phosphoproteins analysis
Rats
Receptors, Cell Surface
Centrosome chemistry
TRPP Cation Channels analysis
Subjects
Details
- Language :
- English
- ISSN :
- 1931-857X
- Volume :
- 291
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- American journal of physiology. Renal physiology
- Publication Type :
- Academic Journal
- Accession number :
- 16609150
- Full Text :
- https://doi.org/10.1152/ajprenal.00282.2005