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More than colocalizing with polycystin-1, polycystin-L is in the centrosome.

Authors :
Bui-Xuan EF
Li Q
Chen XZ
Boucher CA
Sandford R
Zhou J
Basora N
Source :
American journal of physiology. Renal physiology [Am J Physiol Renal Physiol] 2006 Aug; Vol. 291 (2), pp. F395-406. Date of Electronic Publication: 2006 Apr 11.
Publication Year :
2006

Abstract

Polycystin-1 and polycystin-2 are involved in autosomal dominant polycystic kidney disease by unknown mechanisms. These two proteins are located in primary cilia where they mediate mechanosensation, suggesting a link between cilia function and renal disease. In this study, we sought to characterize the subcellular localization of polycystin-L, a closely related member of polycystin-2, in epithelial renal cell lines. We have shown that endogenous polycystin-l subcellular distribution is different in proliferative and nonproliferative cultures. Polycystin-L is found mostly in the endoplasmic reticulum in subconfluent cell cultures, while in confluent cells it is redistributed to sites of cell-cell contact and to the primary cilium as is polycystin-1. Subcellular fractionation confirmed a common distribution of polycystin-L and polycystin-1 in the fractions corresponding to those containing the plasma membrane of postconfluent cells. Reciprocal coimmunoprecipitation experiments showed that polycystin-L was associated with polycystin-1 in a common complex in both subconfluent and confluent cell cultures. Interestingly, we also identified a novel site for a polycystin member (polycystin-L) in unciliated cells, the centrosome, which allowed us to reveal an involvement of polycystin-l in cell proliferation.

Details

Language :
English
ISSN :
1931-857X
Volume :
291
Issue :
2
Database :
MEDLINE
Journal :
American journal of physiology. Renal physiology
Publication Type :
Academic Journal
Accession number :
16609150
Full Text :
https://doi.org/10.1152/ajprenal.00282.2005