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Peptide uptake in the ectomycorrhizal fungus Hebeloma cylindrosporum: characterization of two di- and tripeptide transporters (HcPTR2A and B).
- Source :
-
The New phytologist [New Phytol] 2006; Vol. 170 (2), pp. 401-10. - Publication Year :
- 2006
-
Abstract
- Constraints on plant growth imposed by low availability of nitrogen are a characteristic feature of ecosystems dominated by ectomycorrhizal plants. Ectomycorrhizal fungi play a key role in the N nutrition of plants, allowing their host plants to access decomposition products of dead plant and animal materials. Ectomycorrhizal plants are thus able to compensate for the low availability of inorganic N in forest ecosystems. The capacity to take up peptides, as well as the transport mechanisms involved, were analysed in the ectomycorrhizal fungus Hebeloma cylindrosporum. The present study demonstrated that H. cylindrosporum mycelium was able to take up di- and tripeptides and use them as sole N source. Two peptide transporters (HcPTR2A and B) were isolated by yeast functional complementation using an H. cylindrosporum cDNA library, and were shown to mediate dipeptide uptake. Uptake capacities and expression regulation of both genes were analysed, indicating that HcPTR2A was involved in the high-efficiency peptide uptake under conditions of limited N availability, whereas HcPTR2B was expressed constitutively.
- Subjects :
- Cloning, Molecular
Fungal Proteins genetics
Fungal Proteins metabolism
Gene Expression Regulation, Fungal
Gene Library
Kinetics
Membrane Transport Proteins genetics
Membrane Transport Proteins metabolism
Mutation
Nitrogen metabolism
Phylogeny
Sequence Analysis, Protein
Yeasts genetics
Agaricales metabolism
Dipeptides metabolism
Fungal Proteins physiology
Membrane Transport Proteins physiology
Mycorrhizae metabolism
Oligopeptides metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0028-646X
- Volume :
- 170
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- The New phytologist
- Publication Type :
- Academic Journal
- Accession number :
- 16608464
- Full Text :
- https://doi.org/10.1111/j.1469-8137.2006.01672.x