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Synaptic AMPA receptor exchange maintains bidirectional plasticity.
- Source :
-
Neuron [Neuron] 2006 Apr 06; Vol. 50 (1), pp. 75-88. - Publication Year :
- 2006
-
Abstract
- Activity-dependent synaptic delivery of GluR1-, GluR2L-, and GluR4-containing AMPA receptors (-Rs) and removal of GluR2-containing AMPA-Rs mediate synaptic potentiation and depression, respectively. The obvious puzzle is how synapses maintain the capacity for bidirectional plasticity if different AMPA-Rs are utilized for potentiation and depression. Here, we show that synaptic AMPA-R exchange is essential for maintaining the capacity for bidirectional plasticity. The exchange process consists of activity-independent synaptic removal of GluR1-, GluR2L-, or GluR4-containing AMPA-Rs and refilling with GluR2-containing AMPA-Rs at hippocampal and cortical synapses in vitro and in intact brains. In GluR1 and GluR2 knockout mice, initiation or completion of synaptic AMPA-R exchange is compromised, respectively. The complementary AMPA-R removal and refilling events in the exchange process ultimately maintain synaptic strength unchanged, but their long rate time constants ( approximately 15-18 hr) render transmission temporarily depressed in the middle of the exchange. These results suggest that the previously hypothesized "slot" proteins, rather than AMPA-Rs, code and maintain transmission efficacy at central synapses.
- Subjects :
- Anesthetics, Local pharmacology
Animals
Animals, Newborn
Electric Stimulation methods
Green Fluorescent Proteins metabolism
In Vitro Techniques
Long-Term Potentiation drug effects
Long-Term Potentiation physiology
Long-Term Potentiation radiation effects
Long-Term Synaptic Depression drug effects
Long-Term Synaptic Depression physiology
Long-Term Synaptic Depression radiation effects
Magnesium pharmacology
Mice
Mice, Knockout
Neuronal Plasticity drug effects
Neuronal Plasticity radiation effects
Neurons drug effects
Neurons radiation effects
Neurons virology
Patch-Clamp Techniques methods
Rats
Receptors, AMPA classification
Receptors, AMPA deficiency
Receptors, AMPA genetics
Synapses drug effects
Synaptic Transmission drug effects
Synaptic Transmission physiology
Synaptic Transmission radiation effects
Tetrodotoxin pharmacology
Time Factors
Transfection methods
Vibrissae innervation
Vibrissae physiology
Brain cytology
Neuronal Plasticity physiology
Neurons physiology
Receptors, AMPA physiology
Synapses metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0896-6273
- Volume :
- 50
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Neuron
- Publication Type :
- Academic Journal
- Accession number :
- 16600857
- Full Text :
- https://doi.org/10.1016/j.neuron.2006.02.027